Defining the integration capacity of embryonic stem cell-derived photoreceptor precursors.

Stem Cells
Emma L WestRobin R Ali

Abstract

Retinal degeneration is a leading cause of irreversible blindness in the developed world. Differentiation of retinal cells, including photoreceptors, from both mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), potentially provide a renewable source of cells for retinal transplantation. Previously, we have shown both the functional integration of transplanted rod photoreceptor precursors, isolated from the postnatal retina, in the adult murine retina, and photoreceptor cell generation by stepwise treatment of ESCs with defined factors. In this study, we assessed the extent to which this protocol recapitulates retinal development and also evaluated differentiation and integration of ESC-derived retinal cells following transplantation using our established procedures. Optimized retinal differentiation via isolation of Rax.GFP retinal progenitors recreated a retinal niche and increased the yield of Crx(+) and Rhodopsin(+) photoreceptors. Rod birth peaked at day 20 of culture and expression of the early photoreceptor markers Crx and Nrl increased until day 28. Nrl levels were low in ESC-derived populations compared with developing retinae. Transplantation of early stage retinal cultures produced...Continue Reading

References

Sep 19, 2002·Biochemical and Biophysical Research Communications·Xing ZhaoIqbal Ahmad
Mar 24, 2004·Seminars in Cell & Developmental Biology·Xian-Jie Yang
Jun 30, 2004·Investigative Ophthalmology & Visual Science·Samuel Shao-Min ZhangXin-Yuan Fu
Jul 1, 2004·PLoS Biology·Seth BlackshawConstance L Cepko
Sep 24, 2004·Gene Therapy·U BlömerU Martin
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Stefan ArnholdUlrich Schraermeyer
Jun 28, 2005·Investigative Ophthalmology & Visual Science·Dianca R GrahamJohn D Ash
Aug 2, 2005·Seminars in Cancer Biology·Anna BirgersdotterIngemar Ernberg
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hanako IkedaYoshiki Sasai
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Masayuki AkimotoAnand Swaroop
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Deepak A LambaThomas A Reh
Nov 10, 2006·Nature·R E MacLarenR R Ali
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Takafumi WatayaYoshiki Sasai
Apr 22, 2009·Neuroscience Letters·Yasuhiko HiramiMasayo Takahashi
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jason S MeyerDavid M Gamm
Nov 6, 2010·Investigative Ophthalmology & Visual Science·M Joseph Phillips, Deborah C Otteson
Apr 9, 2011·Nature·Mototsugu EirakuYoshiki Sasai
Jul 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ahad A RahimSimon N Waddington

❮ Previous
Next ❯

Citations

Aug 10, 2013·Nature Biotechnology·David M Gamm, Lynda S Wright
Sep 21, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·David D Eveleth
Mar 26, 2013·The Journal of Clinical Investigation·Lu CuiYing Jin
Mar 26, 2013·The Journal of Clinical Investigation·Hong OuyangKang Zhang
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amanda C BarberRachael A Pearson
Dec 18, 2013·Stem Cells and Development·Katherine Warre-CornishRachael A Pearson
Aug 27, 2014·Stem Cell Research & Therapy·Kamesh DhamodaranDebashish Das
Sep 11, 2014·Neural Regeneration Research·Honghua YuKin-Sang Cho
Jan 24, 2015·Expert Opinion on Biological Therapy·Andrew ZhengStephen H Tsang
Feb 3, 2016·Molecular Therapy. Nucleic Acids·Andriana G KotiniEirini P Papapetrou
Dec 3, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Shomoukh Al-Shamekh, Jeffrey L Goldberg
Sep 4, 2014·Stem Cells·Tiago Santos-FerreiraMarius Ader
Feb 18, 2016·Stem Cells International·Jin YangXiaorong Li
Mar 12, 2015·Biological & Pharmaceutical Bulletin·Fumitaka Osakada, Masayo Takahashi
Oct 6, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vianney DelplaceMolly Shoichet
Mar 24, 2016·Biomacromolecules·Kristan S WorthingtonAliasger K Salem
Aug 27, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Nafiseh Nafissi, Marianna Foldvari
Jan 15, 2014·Biotechnology Advances·Rachael A Pearson
Jul 16, 2013·Experimental Eye Research·Donika GallinaAndy J Fischer
Feb 11, 2015·Progress in Retinal and Eye Research·Sujatha A JayakodyRachael A Pearson
Oct 10, 2013·Progress in Retinal and Eye Research·S BorooahB Dhillon
Mar 13, 2014·Experimental Eye Research·Kristan S WorthingtonBudd A Tucker
Feb 6, 2015·Translational Vision Science & Technology
Jun 18, 2014·Stem Cell Reports·Sarah DecembriniYvan Arsenijevic
Oct 2, 2012·Vision Research·Ander AnasagastiJavier Ruiz-Ederra
Jun 14, 2016·Stem Cells International·Padma Priya SivanSuresh Kumar
May 28, 2016·Journal of Tissue Engineering and Regenerative Medicine·Anat YanaiKevin Gregory-Evans
Sep 7, 2016·Stem Cells International·Nan WuDong Feng Chen
Nov 5, 2016·Biotechnology Letters·Vishal S SharmaFarlan S Veraitch
Mar 5, 2016·Stem Cells Translational Medicine·Akshayalakshmi SridharJason S Meyer
Nov 29, 2016·PloS One·Gordon F HagermanW Ted Allison

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.