Defining the regulatory elements in the proximal promoter of DeltaNp63 in keratinocytes: Potential roles for Sp1/Sp3, NF-Y, and p63

The Journal of Investigative Dermatology
Rose-Anne RomanoSatrajit Sinha

Abstract

p63, a homolog of the tumor suppressor p53, plays an important role in the formation of stratified epithelium such as those in the epidermis of the skin. The p63 gene gives rise to multiple functionally distinct protein isoforms, including the DeltaNp63 class of isoforms, which lacks the N-terminal transactivation domain and is synthesized from an internal promoter. DeltaNp63 proteins are the predominant isoforms expressed in keratinocytes and are thought to be important for maintenance of the proliferative capacity of these cells. Here, we have examined the transcriptional control mechanisms that govern the expression DeltaNp63 in keratinocytes. We first performed DNase I hypersensitive site mapping and demonstrated that the promoter region of DeltaNp63 is in open chromatin state in keratinocytes. To identify the cis-elements that regulate DeltaNp63, we have performed transient transfection assays in keratinocytes with several DeltaNp63 promoter constructs. This identified a short evolutionarily conserved fragment that harbors most of the transcriptional activity of the DeltaNp63 promoter. Biochemical studies of this element have revealed critical roles for CCAAT-box-binding factor (CBF/NF-Y) and Sp1/Sp3 family of proteins. In...Continue Reading

References

Oct 1, 1994·Current Opinion in Genetics & Development·E Fuchs, C Byrne
Jun 5, 1998·Trends in Biochemical Sciences·S N Maity, B de Crombrugghe
Dec 14, 1999·The Journal of Investigative Dermatology·R ParsaH Green
Mar 14, 2000·Molecular and Cellular Biology·S SinhaE Fuchs
Jul 8, 2000·The Journal of Investigative Dermatology·B A JessenR H Rice
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Aug 4, 2001·Biochemical and Biophysical Research Communications·S IwanoT Kamataki
Jan 31, 2002·Trends in Genetics : TIG·Annie YangFrank McKeon
Jun 19, 2002·Journal of Medical Genetics·H G BrunnerH Van Bokhoven
May 29, 2003·The Journal of Biological Chemistry·Giuseppina CarettiRoberto Mantovani
Dec 19, 2003·Nucleic Acids Research·Christoph D SchmidPhilipp Bucher
Jan 20, 2004·Genes & Development·Maranke I KosterDennis R Roop
Mar 23, 2004·Carcinogenesis·Matthew D Westfall, Jennifer A Pietenpol
May 8, 2004·Nature Reviews. Genetics·Wyeth W Wasserman, Albin Sandelin
Feb 1, 2005·European Journal of Cell Biology·Maranke I Koster, Dennis R Roop
Apr 16, 2005·Molecular and Cellular Biology·Carol ImbrianoRoberto Mantovani
Jul 12, 2005·Oncogene·Giulia FontemaggiGiovanni Blandino

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Citations

Oct 2, 2007·BMC Molecular Biology·Serena BorrelliRoberto Mantovani
Jul 23, 2009·Journal of Cell Science·Ramakrishna KommaganiMadhavi P Kadakia
Jun 17, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Adam PackardJames E Schwob
Jun 29, 2007·The Journal of Investigative Dermatology·Atsushi TakagiHideoki Ogawa
Dec 13, 2006·The Journal of Investigative Dermatology·Rose-Anne RomanoSatrajit Sinha
Jul 19, 2012·Journal of Cellular Physiology·Yi-Jen HsuehJan-Kan Chen
May 22, 2009·The Journal of Investigative Dermatology·Silvia PozziRoberto Mantovani
Nov 5, 2011·Critical Reviews in Biochemistry and Molecular Biology·Diletta DolfiniRoberto Mantovani
Jan 16, 2016·Cell Death and Differentiation·J M RizzoR-A Romano
Oct 30, 2007·Journal of Dermatological Science·Priyadharsini Nagarajan, Satrajit Sinha
May 29, 2013·International Journal of Molecular Sciences·Manal FareaAdam Husein
Mar 6, 2007·Oncogene·M P Deyoung, L W Ellisen
Mar 29, 2011·Cellular & Molecular Biology Letters·Marta NekulovaBorivoj Vojtesek
Sep 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kathryn E YohRon Prywes
Dec 15, 2019·Pharmacology & Therapeutics·Yinglu GuanYejing Ge
Feb 28, 2021·The Journal of Pathology·Zuzana PokornáPhilip J Coates
Jul 3, 2021·Cancers·Eugenia BezzecchiRoberto Mantovani
Dec 6, 2011·Biochimica Et Biophysica Acta·Carol ImbrianoRoberto Mantovani

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