Defining the salt effect on human RAD51 activities

DNA Repair
Kang-Sup ShimRichard Fishel

Abstract

Previous work by Sung and colleagues identified unusual salt requirements for hRAD51 strand exchange compared to RecA [S. Sigurdsson, K. Trujillo, B. Song, S. Stratton, P. Sung, Basis for avid homologous DNA strand exchange by human Rad51 and RPA, J. Biol. Chem. 276 (2001) 8798-8806]. Later studies showed that this salt [(NH4)2SO4] appeared to enhance the ability of hRAD51 to distinguish ssDNA from dsDNA [Y. Liu, A.Z. Stasiak, J.Y. Masson, M.J. McIlwraith, A. Stasiak, S.C. West, Conformational changes modulate the activity of human RAD51 protein, J. Mol. Biol. 337 (2004) 817-827]. The mechanism of this salt effect remains enigmatic. Here, we detail the properties of several neutral salts on hRAD51 activities. We found that the cation identity correlated with the stimulatory effect of these neutral salts on hRAD51 ATPase and strand exchange activities. The salt effect appears to be related to the size of the cation, which may be largely mimicked with the cesium ion. These results are consistent with the hypothesis that stimulating cations induce an important conformation and/or transition state in hRAD51. In the presence of an optimal ammonium-based salt (NaNH4HPO4), hRAD51 mediated strand exchange was successfully performed usi...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Jwang, C M Radding
Jan 23, 1992·Nature·R M StoryT A Steitz
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P MenetskiS C Kowalczykowski
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
May 17, 1984·Nature·P Howard-FlandersA Stasiak
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M M Cox, I R Lehman
Mar 8, 1996·The Journal of Biological Chemistry·Q ShanR B Inman
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·R C GuptaC M Radding
Jan 1, 1997·Progress in Nucleic Acid Research and Molecular Biology·A I Roca, M M Cox
Jan 1, 1997·Annual Review of Biochemistry·S R Sprang
Sep 22, 1999·Molecular and Cellular Biology·C MorrisonS Takeda
Feb 8, 2000·The Journal of Biological Chemistry·E A Namsaraev, P Berg
Aug 1, 2000·Mutation Research·P SungS Van Komen
Jan 1, 2001·The Journal of Biological Chemistry·S SigurdssonP Sung
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·X YuE H Egelman
Feb 13, 2002·The Journal of Biological Chemistry·Gregory Tombline, Richard Fishel
Feb 13, 2002·The Journal of Biological Chemistry·Gregory TomblineRichard Fishel
Feb 13, 2002·The Journal of Biological Chemistry·Gregory TomblineRichard Fishel
Nov 28, 2002·Molecular Cell·Jachen A SolingerWolf-Dietrich Heyer
Oct 7, 2003·Annual Review of Microbiology·Michael M Cox
Mar 23, 2004·Journal of Molecular Biology·Yilun LiuStephen C West
May 5, 2004·The Journal of Biological Chemistry·Kang Sup ShimRichard Fishel

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Citations

Jan 26, 2012·The Journal of Biological Chemistry·Ravindra AmunugamaRichard Fishel
Dec 11, 2008·Nucleic Acids Research·Arabela A GrigorescuPhoebe A Rice
Jun 13, 2013·Nucleic Acids Research·Justin A NorthRichard Fishel
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Yang LiYu Luo
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Sep 12, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Charles EsnaultBenoît Chénais
Jul 3, 2013·DNA Repair·Ravindra AmunugamaRichard Fishel
Apr 9, 2013·Biophysical Journal·Yuichi Kokabu, Mitsunori Ikeguchi
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andrea CandelliErwin J G Peterman
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shyamal SubramanyamMaria Spies
Jan 17, 2020·The EMBO Journal·Federico PaolettiFumiko Esashi
Oct 20, 2006·The Journal of Biological Chemistry·Xinguo QianYu Luo

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