Defining TNF-α- and LPS-induced gene signatures in monocytes to unravel the complexity of peripheral blood transcriptomes in health and disease.

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Biljana SmiljanovicRia Baumgrass

Abstract

Several genome-wide transcriptome studies have shown that chronic inflammatory responses generally taking place in the inflamed tissue are also reflected at the level of peripheral blood leukocytes. Blood monocytes are highly sensitized cell type continuously activated under inflammatory conditions. For a better understanding of the transcriptional imprinting influenced by a multitude of pro- and anti-inflammatory mediators, we established a whole blood in vitro system to explore cell- and stimulus-specific gene expression signatures in peripheral monocytes. In an explorative study, whole blood from healthy donors was stimulated with tumour necrosis factor-alpha (TNF-α) or lipopolysaccharide (LPS) for 1.5 h. Subsequently, monocytes were isolated with a purity of >99% by high-speed fluorescence activated cell sorting. Transcriptional changes were explored by whole genome Affymetrix arrays using highly validated filtering algorithm to identify differentially expressed genes. In vitro stimulation of whole blood samples with TNF-α and LPS resulted in 4,529 and 5,036 differentially expressed genes, respectively. Although both stimuli induced similar inflammatory profiles in monocytes, TNF-α- or LPS-specific gene signatures were char...Continue Reading

References

Jan 26, 1999·Annals of the New York Academy of Sciences·C A Dinarello
Nov 30, 2000·Arthritis Research·R W KinneG R Burmester
Aug 2, 2001·Nature Medicine·P MarrackB L Kotzin
Aug 22, 2001·Trends in Cell Biology·V Baud, M Karin
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin MaasThomas M Aune
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emily C BaechlerTimothy W Behrens
Mar 19, 2003·The Journal of Experimental Medicine·Lynda BennettVirginia Pascual
Nov 25, 2004·Current Opinion in Biotechnology·Peter C TaylorMarc Feldmann
Feb 8, 2005·Trends in Molecular Medicine·Luis Ulloa, Kevin J Tracey
Feb 15, 2005·Annals of the Rheumatic Diseases·D J VealeO FitzGerald
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·A Karolina PaluckaJacques Banchereau
Jun 24, 2005·Genes and Immunity·F M BatliwallaP K Gregersen
Aug 19, 2005·The Journal of Biological Chemistry·Karl BalabanianFrançoise Bachelerie
Sep 15, 2006·Genes and Immunity·A K StoeckmanE J Peterson
Sep 19, 2006·Immunity·Jacques Banchereau, Virginia Pascual
Oct 14, 2006·Nature Reviews. Immunology·Richard S Hotchkiss, Donald W Nicholson
Jan 5, 2007·Genome Biology·Albert ZlotnikHisayuki Nomiyama
May 5, 2007·Nature Medicine·Roberto BaccalaArgyrios N Theofilopoulos
May 12, 2007·Cytokine & Growth Factor Reviews·Ian A Clark
May 26, 2007·Nature Reviews. Immunology·Iain B McInnes, Georg Schett
Jun 26, 2007·Clinical and Experimental Immunology·H E BarksbyJ J Taylor
Sep 8, 2007·Deutsche medizinische Wochenschrift·A GrützkauA Radbruch
Dec 25, 2007·Pharmacology & Therapeutics·Daniel TraceyPaul P Tak
Apr 25, 2008·Current Protocols in Immunology·Larry M WahlPhillip D Smith

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Citations

May 23, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Biljana SmiljanovicAndreas Grützkau
Oct 1, 2011·Zeitschrift für Rheumatologie·R Biesen, T Häupl
Jul 17, 2015·Arthritis Research & Therapy·Kristina ConradUta Syrbe
Oct 7, 2014·Molecular Nutrition & Food Research·Danielle L FolkardRichard F Mithen
Nov 2, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Federico MoriconiGiuliano Ramadori
Apr 9, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Till SörensenThomas Häupl
Sep 1, 2020·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Leila HosseinzadehMasoud Sadeghi

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