Definition and validation of operating equations for poly(vinyl alcohol)-poly(lactide-co-glycolide) microfiltration membrane-scaffold bioreactors.

Biotechnology and Bioengineering
R J ShipleyMarianne J Ellis

Abstract

The aim of this work is to provide operating data for biodegradable hollow fiber membrane bioreactors. The physicochemical cell culture environment can be controlled with the permeate flowrate, so this aim necessitates the provision of operating equations that enable end-users to set the pressures and feed flowrates to obtain their desired culture environment. In this paper, theoretical expressions for the pure water retentate and permeate flowrates, derived using lubrication theory, are compared against experimental data for a single fiber poly(vinyl alcohol)-poly(lactide-co-glycolide) crossflow module to give values for the membrane permeability and slip. Analysis of the width of the boundary layer region where slip effects are important, together with the sensitivity of the retentate and permeate equations to the slip parameter, show that slip is insignificant for these membranes, which have a mean pore diameter of 1.1 microm. The experimental data is used to determine a membrane permeability, of k = 1.86 x 10(-16) m(2), and to validate the model. It was concluded that the operating equation that relates the permeate to feed ratio, c, lumen inlet flowrate, Q (l,in), lumen outlet pressure, P (1), and ECS outlet pressure, P (0...Continue Reading

References

Aug 17, 1991·Lancet·E De Clercq
Jan 1, 1974·Biorheology·A ApelblatA Silberberg
Aug 9, 2006·Biotechnology and Bioengineering·Marianne J Ellis, Julian B Chaudhuri
Apr 9, 2008·Archives of Biochemistry and Biophysics·Jonathan I Dawson, Richard O C Oreffo
Jul 1, 1986·Biotechnology and Bioengineering·J P Tharakan, P C Chau
Aug 30, 2008·Biotechnology and Bioengineering·Marianne J Ellis, Julian B Chaudhuri
Nov 13, 2008·Cytotechnology·Robert James ThomasDavid J Williams

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Citations

Jul 30, 2014·Biotechnology Letters·Nelly WungMarianne J Ellis
Mar 4, 2011·Biotechnology and Bioengineering·R J ShipleyM J Ellis
Sep 13, 2014·Biomechanics and Modeling in Mechanobiology·Natalie C PearsonRebecca J Shipley
Jan 17, 2017·Journal of Theoretical Biology·Lloyd A C ChapmanRebecca J Shipley
Feb 27, 2016·The International Journal of Artificial Organs·Hadis EghbaliManuela T Raimondi
Mar 6, 2019·Journal of Tissue Engineering·Iva BurovaRebecca J Shipley
May 18, 2019·Journal of Theoretical Biology·Ian SorrellSteven Webb

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Methods Mentioned

BETA
chemical treatment

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