PMID: 2485081Jan 1, 1989Paper

Definition, at the molecular level, of a thyroglobulin-acetylcholinesterase shared epitope: study of its pathophysiological significance in patients with Graves' ophthalmopathy

Autoimmunity
M LudgateH Soreq

Abstract

The nature of the putative autoantigen in Graves' ophthalmopathy (Go) remains an enigma but the sequence similarity between thyroglobulin (Tg) and acetylcholinesterase (ACHE) provides a rationale for epitopes which are common to the thyroid gland and the eye orbit. In an attempt to define the shared epitope, we have screened a lambda gt 11 human thyroid cDNA library using a polyclonal antibody to Torpedo ACHE and isolated two clones, which upon sequencing, were shown to contain Tg segments, corresponding to portions of the C terminal part of the molecule which has a high similarity with ACHE. Having demonstrated the existence of an epitope common to Tg and ACHE, the clones have been further tested and found to be positive in lysis plaque assays with 1/10 sera from patients with Hashimoto's thyroiditis (HT), 8/8 from patients with Graves' ophthalmopathy and 0/8 normal sera. We have investigated the physiological significance of this common epitope by in situ immunolocalization studies in which the polyclonal antibody to Torpedo ACHE (which was used for screening the library) and immunoglobulins (Igs) from 6 Go patients tested were shown to bind to end plate regions of human foetal muscle fibres which were concurrently shown to b...Continue Reading

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Citations

Aug 1, 1994·Molecular Immunology·M F PrummelS M McLachlan
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette
Mar 6, 2002·Clinical and Experimental Immunology·M Ludgate, G Baker
Sep 22, 1999·European Journal of Biochemistry·D GrisaruH Soreq
Apr 6, 2001·Thyroid : Official Journal of the American Thyroid Association·B Rapoport, S M McLachlan
Aug 17, 2004·Thyroid : Official Journal of the American Thyroid Association·Sandra M McLachlan, Basil Rapoport
Jan 1, 1991·Thyroid : Official Journal of the American Thyroid Association·M SalviJ R Wall
Mar 1, 1992·Journal of Internal Medicine·P Perros, P Kendall-Taylor
Nov 28, 2009·Journal of Neuroimmunology·Carlo ProvenzanoEmanuela Bartoccioni
Oct 1, 1993·Trends in Endocrinology and Metabolism : TEM·P Perros, P Kendall-Taylor
Dec 13, 2003·Endocrine Reviews·Bellur S PrabhakarTerry J Smith
Jul 1, 1993·Journal of Endocrinological Investigation·R EliseiM Ludgate

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