PMID: 9420242Jan 7, 1998Paper

Definition of a 14-amino-acid peptide essential for the interaction between the murine leukemia virus amphotropic envelope glycoprotein and its receptor

Journal of Virology
J L BattiniJ M Heard

Abstract

Hydrophilic loops in the receptor binding domain of the amphotropic murine leukemia virus (MLV) envelope glycoprotein (SU) are predicted and may participate in SU-receptor interactions. We have replaced five segments of 6 to 15 amino acids located in each of these regions with an 11-amino-acid tag from the vesicular stomatitis virus glycoprotein (VSV-G). Substitution was compatible with envelope processing, transport, and incorporation into virions. However, three substitution mutants showed a temperature-dependent phenotype, suggesting structural unstability. Accessibility of the tagging epitope for a monoclonal anti-VSV-G antibody was greater in oligomeric than in monomeric SUs when insertion was done in VRA, a domain essential for receptor recognition. In contrast, accessibility was independent of structural constraints when insertion was done in VRB, a domain playing an accessory role in receptor binding. Interaction with the amphotropic receptor was investigated by interference assay and study of binding and infection of target cells with MLV particles coated with the substituted envelopes. Envelope-receptor interaction was abolished when substitution was performed in a potential loop-forming segment located at the N-termi...Continue Reading

References

Mar 1, 1986·Journal of Virology·A PinterS Silagi
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·N V SomiaI M Verma
Oct 1, 1995·Journal of Virology·F L CossetS J Russell
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Citations

Aug 17, 2004·Journal of Virology·Shervin BahramiFinn Skou Pedersen
Jul 25, 2006·Yeast·Mary GerminoJoseph Germino
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J L BattiniA D Miller
Dec 7, 2006·Virology Journal·Thomas M HowardSuraiya Rasheed
Feb 12, 2010·Cold Spring Harbor Protocols·Els Verhoeyen, François-Loïc Cosset

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