Definition of a simple statistical parameter for the quantification of orientation in two dimensions: application to cells on grooves of nanometric depths

Acta Biomaterialia
P DavidsonK Anselme

Abstract

Contact guidance is generally evaluated by measuring the orientation angle of cells. However, statistical analyses are rarely performed on these parameters. Here we propose a statistical analysis based on a new parameter sigma, the orientation parameter, defined as the dispersion of the distribution of orientation angles. This parameter can be used to obtain a truncated Gaussian distribution that models the distribution of the data between -90 degrees and +90 degrees. We established a threshold value of the orientation parameter below which the data can be considered to be aligned within a 95% confidence interval. Applying our orientation parameter to cells on grooves and using a modelling approach, we established the relationship sigma=alpha(meas)+(52 degrees -alpha(meas))/(1+C(GDE)R) where the parameter C(GDE) represents the sensitivity of cells to groove depth, and R the groove depth. The values of C(GDE) obtained allowed us to compare the contact guidance of human osteoprogenitor (HOP) cells across experiments involving different groove depths, times in culture and inoculation densities. We demonstrate that HOP cells are able to identify and respond to the presence of grooves 30, 100, 200 and 500 nm deep and that the deeper...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Apr 1, 1995·Journal of Biomedical Materials Research·E T den BraberJ A Jansen
Sep 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L C GerstenfeldW J Landis
May 30, 1997·Science·C S ChenD E Ingber
Mar 24, 1998·Journal of Biomedical Materials Research·T G van KootenA von Recum
Mar 18, 1999·Journal of Biomechanical Engineering·D M Brunette, B Chehroudi
Apr 23, 1999·Biomaterials·R G FlemmingP F Nealey
Jun 24, 1999·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Nov 26, 1999·Journal of Biomedical Materials Research·K AnselmeP Hardouin
Mar 11, 2000·Biomaterials·K Anselme
Jul 6, 2000·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Feb 13, 2001·Current Opinion in Genetics & Development·L Zhu, A I Skoultchi
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·P ClarkM Peckham
Jul 19, 2002·Journal of Biomedical Materials Research·W O SoboyejoJ Ricci
Jan 25, 2003·Tissue Engineering·Karine AnselmePierre Hardouin
Jul 16, 2003·Biochemical and Biophysical Research Communications·Christopher S ChenDonald E Ingber
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Xiong Lu, Yang Leng
Nov 1, 2005·Biomaterials·Karine Anselme, Maxence Bigerelle
Dec 5, 2006·Journal of Materials Science. Materials in Medicine·F S Magdon IsmailA Bendavid
Jan 25, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·J ChenW O Soboyejo
Nov 13, 2007·Journal of Biomedical Materials Research. Part a·Adam W FeinbergAnthony B Brennan
Jan 15, 2008·Biomaterials·Mai T LamShuichi Takayama

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Citations

Jul 16, 2013·Acta Biomaterialia·Ling-Fang TsengJames H Henderson
Jan 17, 2016·Journal of the Mechanical Behavior of Biomedical Materials·J M SobralA E Markaki
Dec 4, 2014·Biomaterials·Pitirat PholpabuChristopher J Bettinger
Apr 26, 2016·Acta Biomaterialia·Jonathan T PhamMarcelo Salierno
Apr 8, 2015·PLoS Computational Biology·Nancy K DrewAnna Grosberg
Oct 28, 2016·Journal of Biomechanical Engineering·Nancy K DrewAnna Grosberg
Nov 11, 2015·Journal of Nanobiotechnology·Udesh DhawanWen-Liang Chen

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