PMID: 9539731May 16, 1998Paper

Definition of amide protection factors for early kinetic intermediates in protein folding

Proceedings of the National Academy of Sciences of the United States of America
W A HouryH A Scheraga

Abstract

Hydrogen-deuterium exchange experiments have been used previously to investigate the structures of well defined states of a given protein. These include the native state, the unfolded state, and any intermediates that can be stably populated at equilibrium. More recently, the hydrogen-deuterium exchange technique has been applied in kinetic labeling experiments to probe the structures of transiently formed intermediates on the kinetic folding pathway of a given protein. From these equilibrium and nonequilibrium studies, protection factors are usually obtained. These protection factors are defined as the ratio of the rate of exchange of a given backbone amide when it is in a fully solvent-exposed state (usually obtained from model peptides) to the rate of exchange of that amide in some state of the protein or in some intermediate on the folding pathway of the protein. This definition is straightforward for the case of equilibrium studies; however, it is less clear-cut for the case of transient kinetic intermediates. To clarify the concept for the case of burst-phase intermediates, we have introduced and mathematically defined two different types of protection factors: one is P struc, which is more related to the structure of the...Continue Reading

References

Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·S W Englander, L Mayne
Oct 15, 1991·Biochemistry·A D Robertson, R L Baldwin
Jul 20, 1990·Journal of Molecular Biology·G W BushnellG D Brayer
Jan 1, 1990·Annual Review of Biochemistry·P S Kim, R L Baldwin
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J B Udgaonkar, R L Baldwin
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H A ScheragaP W Mui
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·A R Fersht
Feb 1, 1995·Journal of Biomolecular NMR·R L Baldwin
Sep 1, 1993·Proteins·Y BaiS W Englander
Sep 1, 1993·Proteins·G P ConnellyS W Englander
Jan 1, 1993·Annual Review of Biochemistry·C R Matthews
Jan 1, 1997·Nature Structural Biology·K A Dill, H S Chan
Jan 1, 1997·Trends in Biochemical Sciences·T E Creighton
Feb 1, 1997·Current Opinion in Structural Biology·H Roder, W Colón
Feb 1, 1997·Current Opinion in Structural Biology·W A EatonJ Hofrichter
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·T R SosnickS W Englander
Aug 1, 1997·Trends in Biochemical Sciences·J ClarkeA R Fersht
Aug 1, 1991·Current Biology : CB·R L Baldwin, H Roder
Jan 1, 1986·Biophysical Journal·M Potschka

❮ Previous
Next ❯

Citations

Oct 29, 1998·Protein Science : a Publication of the Protein Society·G KernS Marqusee
May 11, 2006·Chemical Reviews·Heinrich RoderHong Cheng
Apr 21, 2012·Science·Philipp NeudeckerLewis E Kay
Nov 3, 2012·Biochimica Et Biophysica Acta·Sheila S Jaswal
Aug 26, 1998·Folding & Design·J M Sauder, H Roder
Aug 8, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Harold W Mackenzie, D Flemming Hansen
Apr 27, 2017·Scientific Reports·Biancamaria FarinaNunzianna Doti
Dec 24, 2019·Biophysical Journal·Harshesh BhattNeel Sarovar Bhavesh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
M S Briggs, H Roder
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Zoltán GáspáriAndrás Perczel
Journal of Peptide Science : an Official Publication of the European Peptide Society
Imre Jákli, András Perczel
© 2022 Meta ULC. All rights reserved