Definition of amino acids sufficient for plasma membrane association of CD45 and CD45-associated protein

Biochemistry
E D Cahir McFarlandM L Thomas

Abstract

The transmembrane protein tyrosine phosphatase CD45 functions to activate Src-family member kinase activity in T lymphocytes. The inability to activate p56(lck) in CD45-deficient cells results in a higher threshold of signaling through the T cell receptor. The lymphoid-specific CD45-associated protein, CD45AP, interacts with CD45 through transmembrane interactions. Cells lines and mice deficient in CD45 express CD45AP mRNA, yet the protein is poorly expressed, indicating that CD45 is required for efficient expression of CD45AP. Pulse-chase analysis indicates that CD45 associates with CD45AP within minutes of biosynthesis. Cell surface labeling and coimmunoprecipitation demonstrate that CD45AP associates with surface-expressed CD45. Therefore, CD45AP is localized to the plasma membrane. To further characterize this interaction, chimeric proteins containing mutations in CD45 transmembrane regions were expressed, and their ability to associate with CD45AP was determined. Alanine-scan mutations of the CD45 transmembrane region demonstrate that no single amino acid is essential for the interaction with CD45AP. However, the expression of chimeric transmembrane regions indicates that a minimum of three and a maximum of eight amino aci...Continue Reading

References

Jun 1, 1991·Immunology Today·M RethJ C Cambier
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H L OstergaardI S Trowbridge
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T MustelinA Altman
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Apr 1, 1980·The Journal of Experimental Medicine·A L Glasebrook, F W Fitch
Sep 8, 1995·The Journal of Biological Chemistry·K KitamuraA Takeda
Feb 1, 1994·International Immunology·J T PingelM L Thomas
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E D Cahir McFarlandM L Thomas

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Citations

Jan 22, 2008·Biochemistry. Biokhimii︠a︡·G I KrotovA V Filatov
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K G JohnsonM L Thomas
Jun 28, 2005·FEBS Letters·Chen-Ni ChinDonald M Engelman
Oct 9, 1998·The Journal of Investigative Dermatology·G S WoodA Schwandt
Aug 11, 1998·Immunity·C FuA C Chan
May 13, 1999·The Journal of Biological Chemistry·A VeilletteF G Gervais
Jan 16, 1999·The Journal of Biological Chemistry·J BlasioliM L Thomas

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