Apr 10, 2014

Definition of CD4 Immunosignatures Associated with MTB

Frontiers in Immunology
Cecilia S Lindestam Arlehamn, Alessandro Sette

Abstract

We have recently described the first true genome-wide screen for CD4(+) T-cell reactivity directed against Mycobacterium tuberculosis (MTB) in latent TB-infected individuals. The approach relied on predictions of HLA-binding capacity for a panel of DR, DP, and DQ alleles representative of those most commonly expressed in the general population, coupled with high throughput ELISPOT assays. The results identified hundreds of novel epitopes and antigens, and documented the novel observation that T cells in latent MTB infection are confined to the CXCR3(+)CCR6(+) phenotype and largely directed against three antigenic "islands" within the MTB genome. In parallel, we have made generally available to the scientific community the technical approaches and reagents developed in the process, such as motifs, algorithms, and binding assays for several common HLA class II alleles, and a panel of single allele HLA class II transfected cell lines representative of the most frequent specificities in the general population. Recent efforts have been focused on characterization of epitopes and antigens recognized by patients with active TB and individuals vaccinated with BCG, with the aim of providing the first systematic evaluation of the overlap...Continue Reading

Mentioned in this Paper

T-Lymphocyte
CXCR3 gene
CCR6 protein, human
Chemokine Receptor CXCR3 Measurement
Antigens
Latent Tuberculosis
Alleles
CCR6 gene
Enzyme-Linked Immunospot Assay
Mycobacterium tuberculosis

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