Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines.

Genes, Chromosomes & Cancer
T KnutsenT Ried

Abstract

In defining the genetic profiles in cancer, cytogenetically aberrant cell lines derived from primary tumors are important tools for the study of carcinogenesis. Here, we present the results of a comprehensive investigation of 15 established colorectal cancer cell lines using spectral karyotyping (SKY), fluorescence in situ hybridization, and comparative genomic hybridization (CGH). Detailed karyotypic analysis by SKY on five of the lines (P53HCT116, T84, NCI-H508, NCI-H716, and SK-CO-1) is described here for the first time. The five lines with karyotypes in the diploid range and that are characterized by defects in DNA mismatch repair had a mean of 4.8 chromosomal abnormalities per line, whereas the 10 aneuploid lines exhibited complex karyotypes and a mean of 30 chromosomal abnormalities. Of the 150 clonal translocations, only eight were balanced and none were recurrent among the lines. We also reviewed the karyotypes of 345 cases of adenocarcinoma of the large intestine listed in the Mitelman Database of Chromosome Aberrations in Cancer. The types of abnormalities observed in the cell lines reflected those seen in primary tumors: there were no recurrent translocations in either tumors or cell lines; isochromosomes were the mo...Continue Reading

References

Aug 1, 1994·Genes, Chromosomes & Cancer·F MertensF Mitelman
Jan 30, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K A SmithG R Stark
Jul 1, 1993·Nature Genetics·P S MeltzerJ M Trent
Jan 1, 1995·Advances in Cancer Research·B Dutrillaux
Jul 26, 1996·Science·E SchröckT Ried
Apr 10, 1997·Nature·C LengauerB Vogelstein
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·A J RowanI P Tomlinson
Apr 25, 2000·Cytogenetics and Cell Genetics·R MelcherM Schmid
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·W M Abdel-RahmanP A Edwards
Mar 29, 2002·Genes, Chromosomes & Cancer·Kanji KawaiSteve Goodison
Jul 27, 2002·Science·Richard S Maser, Ronald A DePinho
Aug 21, 2002·The Journal of Experimental Medicine·Michael J DifilippantonioAndré Nussenzweig
Jun 2, 2004·International Journal of Cancer. Journal International Du Cancer·Jordi CampsRosa Miró
Oct 9, 2004·Genes, Chromosomes & Cancer·Jeffrey R SawyerJohn Shaughnessy
Dec 2, 2004·Cancer Genetics and Cytogenetics·Kristine KleiviRagnhild A Lothe
May 7, 2005·International Journal of Clinical Practice. Supplement·G C Beattie, S T Irwin
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Mar 25, 2009·Nature Reviews. Cancer·Christoph A Klein

❮ Previous
Next ❯

Citations

Apr 15, 2011·Journal of Biomedicine & Biotechnology·Lucia MiglioreFabio Coppedè
Jul 21, 2010·Experimental Cell Research·David GisselssonHerman Yeger
Apr 6, 2016·Gastroenterology Research and Practice·Wael M Abdel-RahmanPäivi Peltomäki
Sep 30, 2015·Molecular and Cellular Biology·Aurimas VinckeviciusDebabrata Chakravarti
Feb 7, 2019·Frontiers in Plant Science·Elena Rakosy-TicanAdriana Aurori
Oct 31, 2020·Journal of Cellular and Molecular Medicine·Xueyuan JiaSongbin Fu
Mar 5, 2017·Molecular Cancer Research : MCR·Laura SeamanIndika Rajapakse
Jul 29, 2017·Molecular Cancer Therapeutics·Guido J R ZamanJoost C M Uitdehaag

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.