Definitive N-terminal protein sequence and further characterization of the novel apolipoprotein A5 in human serum

Clinical Chemistry
William E AlbornRobert J Konrad

Abstract

Apolipoprotein A5 (ApoA5) originally gained attention as a regulator of serum triglyceride concentrations through transgenic mouse studies. Our group recently developed the first assay to quantify serum ApoA5 protein concentrations and demonstrated that they are increased by administration of a potent peroxisome proliferator-activated receptor-alpha agonist. To better characterize the circulating ApoA5, the protein was purified from human serum, and a definitive N-terminal protein sequence was obtained. In light of previous observations that ApoA5 was present in VLDL and not LDL, plasma infranatant and intermediate-density lipoprotein (IDL) were analyzed for ApoA5. Because the mature protein contains a single unpaired cysteine, ApoA5 in human serum was immunoprecipitated, and its migration pattern was examined via Western blotting under reducing and nonreducing conditions to determine whether the protein circulates as a disulfide-linked homodimer or heterodimer. Definitive N-terminal protein sequences obtained from ApoA5 purified from human serum indicated that cleavage of the signal peptide occurs in vivo at the predicted site. We found ApoA5 in VLDL, HDL, and chylomicrons but not in LDL, IDL, or plasma infranatant. Under both...Continue Reading

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Citations

Dec 4, 2008·Journal of Lipid Research·Trudy M ForteRobert O Ryan
Jan 12, 2013·Journal of Lipid Research·Elena Mendoza-BarberáFrancisco Blanco-Vaca
Jun 14, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Marinez Oliveira SousaMiguel-Angel Navarro
Dec 26, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·William E AlbornRobert J Konrad
Aug 13, 2011·Atherosclerosis·Stefan K NilssonMartin Merkel
Apr 26, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Akira MatsunagaKeijiro Saku
May 15, 2007·Current Opinion in Lipidology·Kasuen Wong, Robert O Ryan
Apr 3, 2007·The Journal of Biological Chemistry·Jennifer A BecksteadRobert O Ryan
Dec 14, 2019·Journal of Lipid Research·Mark CastleberryW Sean Davidson
Mar 26, 2021·Journal of Lipid Research·Yan Q ChenRobert J Konrad

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