Deformability based sorting of red blood cells improves diagnostic sensitivity for malaria caused by Plasmodium falciparum

Lab on a Chip
Quan GuoHongshen Ma

Abstract

The loss of red blood cell (RBC) deformability is part of the pathology of many diseases. In malaria caused by Plasmodium falciparum infection, metabolism of hemoglobin by the parasite results in progressive reduction in RBC deformability that is directly correlated with the growth and development of the parasite. The ability to sort RBCs based on deformability therefore provides a means to isolate pathological cells and to study biochemical events associated with disease progression. Existing methods have not been able to sort RBCs based on deformability or to effectively enrich for P. falciparum infected RBCs at clinically relevant concentrations. Here, we develop a method to sort RBCs based on deformability and demonstrate the ability to enrich the concentration of ring-stage P. falciparum infected RBCs (Pf-iRBCs) by >100× from clinically relevant parasitemia (<0.01%). Deformability based sorting of RBCs is accomplished using ratchet transport through asymmetrical constrictions using oscillatory flow. This mechanism provides dramatically improved selectivity over previous biophysical methods by preventing the accumulation of cells in the filter microstructure to ensure that consistent filtration forces are applied to each ce...Continue Reading

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Citations

Mar 24, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ning KangMark D Scott
Oct 21, 2016·Lab on a Chip·D CaballeroS Sánchez
Nov 11, 2017·Lab on a Chip·N KolluriM Cabodi
May 24, 2018·International Journal for Numerical Methods in Biomedical Engineering·Sazid Zamal HoqueB S V Patnaik
May 20, 2020·Lab on a Chip·Yuncheng ManUmut A Gurkan
Feb 11, 2020·Frontiers in Physiology·Mallorie DepondPapa Alioune Ndour
Jul 17, 2020·Frontiers in Physiology·Helene Guizouarn, Gregory Barshtein
Nov 13, 2018·Biomechanics and Modeling in Mechanobiology·Matthew BectonXianqiao Wang
Jun 8, 2021·Physical Review Fluids·Xiao ZhangMichael D Graham
Dec 8, 2021·Lab on a Chip·Erik S LamoureuxHongshen Ma

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