Deformation measurement of individual cells in large populations using a single-cell microchamber array chip.

Applied Physics Letters
I DohFrans A Kuypers

Abstract

We analyze the deformability of individual red blood cells (RBCs) using SiCMA technology. Our approach is adequate to quickly measure large numbers of individual cells in heterogeneous populations. Individual cells are trapped in a large-scale array of micro-wells, and dielectrophoretic (DEP) force is applied to deform the cells. The simple structures of micro-wells and DEP electrodes facilitate the analysis of thousands of RBCs in parallel. This unique method allows the correlation of red cell deformation with cell surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous populations.

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Citations

Jan 23, 2013·Journal of Microbiological Methods·Kazuo UmemuraShigeki Mayama
Jan 31, 2017·PLoS Computational Biology·Weikang WangFeng Liu
Oct 27, 2015·Lab on a Chip·Shantimoy KarSuman Chakraborty
Apr 24, 2018·Applied Sciences·E DuJia Liu

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Methods Mentioned

BETA
atomic force microscopy
flowcytometry
chips
chip
fluorescence imaging
imaging technique

Software Mentioned

matlab

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