Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world

The Journal of General Virology
S DubeB J Poiesz

Abstract

Degenerate and specific PCR assays were developed for bovine leukaemia virus (BLV) and/or primate T cell leukaemia/lymphoma viruses (PTLV). The degenerate assays detected all major variants of the BLV/PTLV genus at a sensitivity of 10-100 copies of input DNA; the specific systems detected 1-10 copies of input target. Sensitivity was 100% in specific DNA-PCR assays done on peripheral blood from seropositive BLV-infected cattle and HTLV-I- or HTLV-II-infected humans, and 62% in RNA/DNA-PCR assays on sera from BLV seropositive cattle. The pol fragments from 21 different BLV strains, isolated from cattle in North and Central America, were cloned and sequenced, and compared to other published BLV and PTLV pol sequences. BLV and PTLV sequences differed by 42%. Sequence divergence was up to 6% among the BLV strains, and up to 36% among the PTLV strains (with PTLV-I and PTLV-II differing among themselves by 15% and 8%, respectively). Some cows were infected with several BLV strains. Among retroviruses, BLV and PTLV sequences formed a distinct clade. The data support the interpretation that BLV and PTLV evolved from a common ancestor many millennia ago, and some considerable time before the PTLV-I and PTLV-II strains diverged from each ...Continue Reading

Citations

Jul 10, 2009·The Journal of General Virology·Sabrina M RodriguezLeandro R Jones
Aug 5, 2009·Virology Journal·Syamalima DubeBernard J Poiesz
May 25, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kristin HeenemannThomas W Vahlenkamp
May 22, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·A ZaghawaO Marquardt
Apr 24, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·D MartinE Gómez-Lucía
Aug 19, 2006·Virus Genes·Marcelo Fernandes CamargosRômulo Cerqueira Leite
Feb 11, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·K M McGirr, G C Buehring
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Cécile VoissetDavid J Griffiths
Jul 18, 2000·Journal of Medical Virology·W D AndrewsJ A Garson
Feb 19, 2017·Archives of Virology·Aneta PlutaJacek Kuźmak

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