Degenerin/epithelial Na+ channel proteins: components of a vascular mechanosensor

Hypertension
H DrummondDavid R Harder

Abstract

Mechanosensitive ion channels are thought to mediate stretch-induced contraction in vascular smooth muscle cells (VSMCs); however, the molecular identity of the mechanosensitive ion channel complex is unknown. Although recent reports suggest degenerin/epithelial Na+ channel (DEG/ENaC) proteins may be mechanosensors in sensory neurons, their role as mechanosensors in vascular tissue has not been examined. We first tested whether DEG/ENaC subunits are expressed in cerebral blood vessels and VSMCs and then examined their role as mechanosensors in mediating the myogenic response in intact blood vessels. Using RT-PCR, we found ENaC transcripts expressed in rat cerebral arteries and freshly dissociated rat cerebral VSMCs. We also detected ENaC expression in isolated blood vessels and VSMCs by immunoblotting and immunolocalization. Moreover, inhibition of ENaC with amiloride (1 micromol/L) and benzamil (30 nmol/L, 1 micromol), an amiloride analog, blocked myogenic constriction in isolated rat cerebral arteries. These data suggest that DEG/ENaC proteins are required for vessel responses to pressure and are consistent with the evolutionary conservation of mechanosensory function of DEG/ENaC proteins.

References

Apr 1, 1992·The American Journal of Physiology·M J DavisJ D Hood
Sep 1, 1992·The American Journal of Physiology·G A Meininger, M J Davis
Feb 1, 1990·The Journal of Membrane Biology·C E Morris
Jan 1, 1986·Membrane Biochemistry·F Sachs
Oct 1, 1988·The Journal of Membrane Biology·T R Kleyman, E J Cragoe
Jun 1, 1995·The American Journal of Physiology·M S AwaydaD J Benos
May 1, 1995·The American Journal of Physiology·F J McDonaldM J Welsh
Jan 12, 1996·The Journal of Biological Chemistry·I I IsmailovD J Benos
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·N KizerK Hruska
Mar 13, 1997·Nature·R WaldmannM Lazdunski
Aug 5, 1998·The Journal of General Physiology·M S Awayda, M Subramanyam
Oct 23, 1998·Nature·S UgawaS Shimada
Nov 14, 1998·The American Journal of Physiology·H L JiD J Benos
Apr 30, 1999·Physiological Reviews·M J Davis, M A Hill
Jul 10, 1999·The Journal of General Physiology·S KellenbergerL Schild
Sep 3, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·I Mano, M Driscoll
Oct 13, 2000·Fundamental & Clinical Pharmacology·A OyabeK Nakayama
Dec 21, 2000·American Journal of Physiology. Cell Physiology·Y MutoT Urushidani
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·X Wu, M J Davis
Aug 21, 2001·Pflügers Archiv : European journal of physiology·F SchwedaA Kurtz
Aug 1, 2002·The Journal of General Physiology·Ahmed Chraïbi, Jean-Daniel Horisberger
Oct 19, 2002·Journal of Neuroscience Methods·Ellicia F CoyneH Richard Winn
Nov 20, 2003·The Journal of Biological Chemistry·Marcelo D CarattinoThomas R Kleyman

❮ Previous
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Citations

Oct 24, 2007·The Journal of Membrane Biology·Shi-Peng WeiHe-Ping Ma
May 24, 2005·Pflügers Archiv : European journal of physiology·Roger G O'Neil, Stefan Heller
Oct 6, 2006·Pflügers Archiv : European journal of physiology·O P Hamill
Sep 18, 2007·Pflügers Archiv : European journal of physiology·Martin Fronius, Wolfgang G Clauss
May 31, 2011·Acta Pharmacologica Sinica·Yan SunZhi-ren Zhang
May 29, 2012·Cardiovascular Research·Gilles KauffensteinDaniel Henrion
Dec 25, 2007·Exercise and Sport Sciences Reviews·Philip S Clifford, Michael E Tschakovsky
May 29, 2013·Clinical and Experimental Pharmacology & Physiology·Cara J Büsst
Nov 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Adebowale AdebiyiJonathan H Jaggar
Jun 2, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikki L JerniganThomas C Resta
Jun 26, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lauren G VanLandinghamHeather A Drummond
May 23, 2008·American Journal of Physiology. Renal Physiology·Xuemei WangRodger Loutzenhiser
Jul 15, 2011·American Journal of Physiology. Renal Physiology·Ossama B Kashlan, Thomas R Kleyman
Nov 6, 2009·American Journal of Physiology. Renal Physiology·Samira C GrifoniHeather A Drummond
Nov 10, 2011·Hypertension·Michael J RyanHeather A Drummond
Jan 1, 2014·Current Opinion in Nephrology and Hypertension·Kristina Kusche-VihrogFrédéric Jaisser
Jan 15, 2014·Nature Reviews. Nephrology·David G WarnockFrederic Jaisser
Nov 17, 2009·The Journal of Membrane Biology·Silvana M Del MónacoBasilio A Kotsias
Jun 15, 2006·The Journal of Membrane Biology·A J RicciS M Van Netten
Aug 12, 2014·Pharmacology & Therapeutics·Andrea OlschewskiHorst Olschewski
Mar 28, 2012·Molecular Pain·Ramin RaoufJohn N Wood
Mar 13, 2012·Experimental Cell Research·Ossama B Kashlan, Thomas R Kleyman
Jun 23, 2009·Trends in Pharmacological Sciences·Michael A HillIsmail Laher
Mar 18, 2008·Progress in Biophysics and Molecular Biology·Joost H A FolgeringEric Honoré
Oct 15, 2013·The Journal of Membrane Biology·Luciano GaliziaBasilio A Kotsias
Jul 19, 2005·Current Opinion in Neurobiology·Ellen A Lumpkin, Diana M Bautista
Aug 25, 2015·American Journal of Hypertension·Yu-Long LiRobert L Muelleman
Jan 10, 2006·Developmental Cell·A Wayne OrrMartin A Schwartz
Oct 1, 2015·Nephrology·Visith Sitprija, Siravit Sitprija
Jul 28, 2013·Biochimica Et Biophysica Acta·Boris Martinac
Aug 6, 2016·BioMed Research International·Silvia Chifflet, Julio A Hernandez
Aug 26, 2016·Clinical and Experimental Pharmacology & Physiology·Lori S KangMatthew A Boegehold
Jun 28, 2008·American Journal of Hypertension·Haidong ZhuYanbin Dong

❮ Previous
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