Deglycosylation of Toxocara excretory-secretory antigens improves the specificity of the serodiagnosis for human toxocariasis

Parasite Immunology
William Henry RoldánAntonio Walter Ferreira

Abstract

Serodiagnosis of human toxocariasis is difficult in tropical areas where other helminthiasis are endemic. Many studies have shown that glycans from helminths may be the responsible for cross-reactions in the immunoassays. In this study, we have evaluated the deglycosylation of the Toxocara canis excretory-secretory (TES) antigens for the detection of IgG antibodies using a panel of 228 serum samples (58 patients with toxocariasis, 75 patients with other helminth infections and 95 healthy individuals) by ELISA and Western blot assays. Our results showed that the deglycosylation of TES antigens resulted in a single fraction of 26 kDa (dTES) and was able to detect IgG antibodies with a sensitivity and specificity of 100% in both above-mentioned assays. The rate of cross-reactions, observed in ELISA with TES (13·3%), was significantly reduced (5·3%) when the dTES antigens were used. Likewise, the cross-reactivity observed with the fractions of 32, 55 and 70 kDa of the TES antigens was totally eliminated when the dTES were used in the Western blot. All these results showed that the deglycosylation of the TES antigens really improves the specificity of the serodiagnosis of human toxocariasis in endemic areas for helminth infections.

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Citations

May 26, 2017·Parasite Immunology·W H RoldánA W Ferreira
Jun 26, 2018·Parasite Immunology·Lucas Moreira Dos SantosFabricio Rochedo Conceição
Nov 9, 2018·Transactions of the Royal Society of Tropical Medicine and Hygiene·Somayeh AghamolaieAli Rostami
Dec 20, 2019·PLoS Neglected Tropical Diseases·Ali RostamiRobin B Gasser
Aug 15, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ali RostamiRobin B Gasser
Apr 6, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Fatemeh CheraghaliMitra Sharbatkhori
May 28, 2021·Research and Reports in Tropical Medicine·Pedro Paulo ChieffiSergio Vieira Dos Santos
Jun 10, 2020·Infectious Disorders Drug Targets·Negar AsadiShahram Khademvatan

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