Degradation of 2,4-dichlorophenoxyacetic acid by Pseudomonas cepacia DBO1(pRO101) in a dual-substrate chemostat.

Applied and Environmental Microbiology
D D Daugherty, S F Karel

Abstract

To determine the effect of a secondary carbon source on biodegradation of a chloroaromatic compound, Pseudomonas cepacia DBO1(pRO101) was grown in continuous cultures on basal salts media containing various mixtures of 2,4-dichlorophenoxyacetic acid (2,4-D) and succinate. Both succinate and 2,4-D were metabolized over the entire range of dilution rates and compositions analyzed (0.05 to 0.6 h-1). 2,4-Dichlorophenol (DCP), the only intermediate detected, accumulated to significant amounts (10 to 21 mg/liter) in the chemostat only when the dilution rate was 0.4 h-1 or greater. At these concentrations, DCP reduced the apparent growth rate of P. cepacia DBO1(pRO101) in batch cultures by 15 to 35% over the apparent growth rate on succinate alone. Succinate fed to the chemostat increased the cell density as well as the percentage of 2,4-D that was consumed at each dilution rate. When the amount of succinate in the feed exceeded the amount of 2,4-D, the specific rates of 2,4-D degradation in the chemostat or by washed cells were significantly lower than the specific rates for cells grown on 2,4-D alone, suggesting repression by succinate. However, when the amount of 2,4-D in the feed exceeded the amount of succinate, the specific rate...Continue Reading

References

Apr 1, 1990·Ecotoxicology and Environmental Safety·M Nendza, J K Seydel
Jan 1, 1988·Annual Review of Microbiology·W Reineke, H J Knackmuss
Jan 1, 1988·Basic Life Sciences·T D FrickG Bateson
Jul 1, 1982·Applied and Environmental Microbiology·J J KilbaneA M Chakrabarty
Sep 1, 1982·Biotechnology and Bioengineering·A C Papanastasiou, W J Maier
Nov 1, 1985·Biotechnology and Bioengineering·E J Bouwer, P L McCarty
Jan 15, 1989·Biotechnology and Bioengineering·R J Machado, C P Grady
Sep 1, 1969·Journal of Agricultural and Food Chemistry·J M TiedjeJ E Dawson

❮ Previous
Next ❯

Citations

Sep 16, 2011·Pest Management Science·Christophe CalvayracJean-François Cooper
May 21, 2010·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Maria G Ziagova, Maria Liakopoulou-Kyriakides
Jun 13, 2015·Applied Microbiology and Biotechnology·Lan Huong Nguyen, Nyuk-Min Chong
May 29, 2012·Environmental Technology·Sumaya Ferreira-GuedesAna Lúcia Leitão
May 16, 2001·Biotechnology and Bioengineering·S A JackmanC J Knowles
Oct 1, 1996·Applied and Environmental Microbiology·K O'ConnorA D Dobson
Jul 1, 1997·Applied and Environmental Microbiology·F AmpeN D Lindley
Apr 1, 1997·Applied and Environmental Microbiology·J DunbarL Forney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.