Degradation of 4-Chlorophenol via the meta Cleavage Pathway by Comamonas testosteroni JH5.

Applied and Environmental Microbiology
J HollenderW Dott

Abstract

Comamonas testosteroni JH5 used 4-chlorophenol (4-CP) as its sole source of energy and carbon up to a concentration of 1.8 mM, accompanied by the stoichiometric release of chloride. The degradation of 4-CP mixed with the isomeric 2-CP by resting cells led to the accumulation of 3-chlorocatechol (3-CC), which inactivated the catechol 2,3-dioxygenase. As a result, further 4-CP breakdown was inhibited and 4-CC accumulated as a metabolite. In the crude extract of 4-CP-grown cells, catechol 1,2-dioxygenase and muconate cycloisomerase activities were not detected, whereas the activities of catechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde hydrolase, and 2-oxopent-4-enoate hydratase were detected. These enzymes of the meta cleavage pathway showed activity with 4-CC and with 5-chloro-2-hydroxymuconic semialdehyde. The activities of the dioxygenase and semialdehyde dehydrogenase were constitutive. Two key metabolites of the meta cleavage pathway, the meta cleavage product (5-chloro-2-hydroxymuconic semialdehyde) and 5-chloro-2-hydroxymuconic acid, were detected. Thus, our previous postulation that C. testosteroni JH5 uses the meta cleavage pathway for the complete mineralization of 4-CP...Continue Reading

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Citations

Jul 1, 2009·International Journal of Molecular Sciences·Kei ShimodaHiroki Hamada
Jan 30, 2008·Applied Microbiology and Biotechnology·Maria Isabel M FerreiraDick B Janssen
Mar 8, 2006·Applied Microbiology and Biotechnology·George P Prpich, Andrew J Daugulis
Oct 20, 2009·Applied and Environmental Microbiology·Maria Isabel M FerreiraToshiaki Kudo
Mar 6, 2019·Frontiers in Microbiology·Yitayal Shiferaw Anteneh, Christopher Milton Mathew Franco
Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Aug 19, 2014·Microbial Biotechnology·Daniel Dobslaw, Karl-Heinrich Engesser
Sep 24, 2013·Environmental Science and Pollution Research International·Pankaj Kumar AroraVijay Pal Singh
Nov 6, 2003·Microbiology·Helia RadianingtyasAlan T Bull
Dec 15, 2010·Environmental Health Insights·Kei Shimoda, Hiroki Hamada

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