Degradation of annexin I in bronchoalveolar lavage fluid from patients with cystic fibrosis

American Journal of Respiratory Cell and Molecular Biology
F H TsaoJ Hu

Abstract

Annexin I is a 36 kilodalton (kD) calcium-dependent phospholipid-binding protein which may have anti-inflammatory properties. Previous investigations which sampled lower respiratory tract epithelial lining fluid (ELF) via bronchoalveolar lavage (BAL) have demonstrated that annexin I can be degraded in inflammatory lung disease. We analyzed BAL fluid from patients with cystic fibrosis (CF) to determine the effects of lung inflammation on the structure and activity of annexin I. Intact annexin I was absent in 17 out of 20 BAL fluid samples from patients with CF, due largely to degradation to a 33 kD protein. The three CF BAL fluids in which annexin I was detectable had very little or no unopposed neutrophil elastase activity in contrast to the 17 in which no annexin I was detectable. Annexin I was present in all BAL fluid samples from 10 normal volunteer (NV) subjects and 12 patients with interstitial lung disease (ILD). The 33 kD annexin I breakdown product was not detectable in samples from NV, but was detectable only in ILD patients with relatively high percentages of neutrophils on BAL differential cell counts. Annexin I appeared to be cleaved by neutrophil elastase at the N-terminal portion between Val-36 and Ser-37 to yield...Continue Reading

References

May 1, 1992·Bone and Mineral·E RojasH B Pollard
Nov 6, 1992·Science·C E Creutz
Jan 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M P AmbroseG W Hunninghake
Jan 1, 1991·Methods in Enzymology·R B Pepinsky
Jan 1, 1991·Medical Microbiology and Immunology·J Römisch, E P Pâques
Jan 1, 1991·British Journal of Pharmacology·J DavidsonD Rotondo
Feb 7, 1991·Biochimica Et Biophysica Acta·F H TsaoP M DeLuca
Apr 1, 1990·Journal of Applied Physiology·M P Ambrose, G W Hunninghake
Oct 1, 1990·American Journal of Respiratory Cell and Molecular Biology·M P Ambrose, G W Hunninghake
May 22, 1990·Biochemistry·N C KhannaD M Waisman
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G CirinoR B Pepinsky
Apr 1, 1989·Biochemical Society Transactions·R Flower
Aug 1, 1989·British Journal of Pharmacology·M Errasfa, F Russo-Marie
Jun 30, 1988·Biochemical and Biophysical Research Communications·M Errasfa, F Russo-Marie
Apr 1, 1996·The American Journal of Physiology·L LiuU J Zimmerman

❮ Previous
Next ❯

Citations

Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Anne VankeerberghenJean-Jacques Cassiman
Aug 8, 2007·The Journal of Biological Chemistry·Linda VongMauro Perretti
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Clifford C TaggartNoel G McElvaney
Oct 21, 2011·BMC Immunology·Amílcar S DamazoSonia M Oliani
Mar 5, 2014·Disease Markers·Shuk-Man KaAnn Chen
Dec 3, 2014·Pharmacology & Therapeutics·Chengxue QinRebecca H Ritchie
Feb 18, 2016·Journal of Immunology Research·Michelle Amantéa SugimotoLirlândia Pires Sousa
Oct 27, 2010·International Immunopharmacology·Lydia SpurrFulvio D'Acquisto
Jun 16, 2010·Pharmacology & Therapeutics·Neil Dufton, Mauro Perretti
Sep 20, 2015·Biochemical Pharmacology·Angela A SenaSilvia G Correa
Jul 22, 2008·British Journal of Pharmacology·F D'AcquistoR J Flower
Apr 30, 2005·Immunology Letters·Mari GotohIsamu Matsumoto
Sep 1, 2004·Biochemical and Biophysical Research Communications·Matthew J Hayes, Stephen E Moss
Oct 7, 2006·Biochimica Et Biophysica Acta·Ursula RescherVolker Gerke
Feb 26, 2013·Biochemical Pharmacology·Fulvio D'AcquistoLorenza Rattazzi
Feb 13, 2001·The American Journal of Pathology·S M OlianiM Perretti
Dec 19, 2015·American Journal of Respiratory Cell and Molecular Biology·Luciana P TavaresLirlândia P Sousa
Jul 23, 2016·Biological Chemistry·Thomas Gobbetti, Sadani N Cooray
Feb 18, 2004·Journal of Leukocyte Biology·Mauro Perretti, Roderick John Flower
May 3, 2018·Physiological Reviews·Nandini KrishnamoorthyBruce D Levy
Oct 29, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katherine R MartinVéronique Witko-Sarsat
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Bénédicte CauweGhislain Opdenakker
Oct 4, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A A S SenaS M Oliani
Mar 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Susan D ReynoldsBarry R Stripp
Jan 19, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gary R MartinJohn L Wallace
Jan 2, 2009·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Deepak K BhallaC Gary Gairola
Aug 9, 2017·Expert Opinion on Therapeutic Targets·Luiza Oliveira PerucciLirlândia Pires Sousa
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Egle SolitoMauro Perretti
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eduard B BabiychukAnnette Draeger
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robert HannonRoderick J Flower
Aug 11, 2012·The Journal of Biological Chemistry·Josh C WoloszynekChristine T N Pham
Jan 24, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juliana P VagoLirlândia P Sousa
Apr 10, 2017·Immunologic Research·Luiza Oliveira PerucciLirlândia Pires Sousa
Aug 28, 2020·Frontiers in Pharmacology·Antonio RecchiutiMario Romano
Mar 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael ScannellPaola Maderna

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.