Degradation of C/EBPbeta in cultured myotubes is calpain-dependent

Journal of Cellular Physiology
Wei WeiPer-Olof Hasselgren

Abstract

Members of the C/EBP transcription factor family regulate cell differentiation and multiple other cellular functions. The cellular levels of C/EBPalpha, gamma, delta, epsilon, and Gadd153/CHOP are regulated in part by proteasome-dependent degradation. In contrast, mechanisms regulating the degradation of C/EBPbeta are poorly understood. We tested the hypothesis that the degradation of C/EBPbeta is calpain-dependent. Studies were performed in cultured L6 myotubes (a rat skeletal muscle cell line) because we have found previously that C/EBPbeta may be involved in the regulation of muscle proteolysis. Treatment of cultured L6 myotubes with the calpain inhibitors calpeptin and Calpain Inhibitor I and II resulted in increased C/EBPbeta concentrations but did not influence cellular levels of the other C/EBP transcription factor family members. Transfection of myoblasts with a plasmid expressing the endogenous calpain inhibitor calpastatin resulted in increased cellular levels of C/EBPbeta whereas the opposite result was observed in myoblasts overexpressing micro- or m-calpain. Co-immunoprecipitation provided evidence for protein-protein interaction between C/EBPbeta and micro- and m-calpain suggesting that C/EBPbeta may be a calpain ...Continue Reading

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Citations

May 19, 2010·Molecular and Cellular Biology·Calin-Bogdan ChiribauMaria Hatzoglou
Feb 2, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Wei ChamberlainPer-Olof Hasselgren
May 22, 2008·American Journal of Physiology. Endocrinology and Metabolism·Ira J SmithPer-Olof Hasselgren
Jan 18, 2007·BMC Medical Genetics·María E SáezManuel Serrano-Ríos
Mar 31, 2009·Future Microbiology·Yujie LiuBrian Wigdahl
Nov 19, 2008·Cellular Signalling·Louise CarneyAnthony D Whetton
Mar 1, 2012·Biochemical and Biophysical Research Communications·Yuan-yuan ZhangXi Li
Mar 1, 2012·Cellular Signalling·René HuberKorbinian Brand
Jun 14, 2016·Archives of Oral Biology·Tewarat KumchantuekShoichi Iseki
Oct 8, 2016·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Dahye YoonSuhkmann Kim
Sep 18, 2010·Clinical and Experimental Immunology·C TabataT Nakano
Jul 20, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Natasha ReillyPer-Olof Hasselgren
Nov 30, 2018·Current Medicinal Chemistry·Bhawana Sharma, Rajesh Dabur
Mar 19, 2016·Physiological Research·Jiaru Huang, Xiaoping Zhu

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