Degradation of double-stranded xanthan by hydrogen peroxide in the presence of ferrous ions: comparison to acid hydrolysis

Carbohydrate Research
B E ChristensenO Smidsrød

Abstract

Conformationally ordered, double-stranded xanthan, degraded in the presence of H2O2 and Fe2+ (at 20 degrees C) or in dilute acid (0.1 M HCl at 80 degrees C), produced xanthan variants with weight-average molecular weights (Mw) ranging from 2 x 10(6) to 5.4 x 10(4). In both cases the fraction of cleaved linkages in the glucan backbone (alpha), measured as reducing ends, increased to very high values (0.05 for Mw = 2-3 x 10(4)), demonstrating that a large number of linkages in the backbone could be cleaved without a correspondingly large reduction in Mw, in accordance with the double-stranded nature of xanthan. Extensive degradation (more than 10-fold reduction in Mw) in both cases released single-stranded, conformationally disordered oligomers; this release was accompanied by an increase in the rate of acid hydrolysis of the glucan backbone and a pronounced increase in the rate of release of glucose monomer. In contrast, there was no significant change in the rate of reducing end-group formation associated with the release of oligomers upon degradation with H2O2/Fe2+. Both types of degradation were accompanied by changes in the composition of the side chains. However, in contrast to acid hydrolysis, where the terminal beta-D-man...Continue Reading

References

Nov 14, 2001·Infection and Immunity·S HaranagaY Yamamoto

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Citations

Jun 16, 2005·International Journal of Pharmaceutics·Hagen Trommer, Reinhard H H Neubert
Jun 5, 2004·Journal of Hazardous Materials·Elisabeth NeyensBart De heyder
May 17, 2014·Current Eye Research·Carla AmicoAnna Rita Blanco
Aug 4, 2015·Chemosphere·Asha SrinivasanKwang V Lo
Jul 25, 2017·Carbohydrate Polymers·Bahtiyor MuhitdinovAbbaskhan Turaev
Jul 5, 2021·International Journal of Biological Macromolecules·Tahreem RiazJingjing Chen

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