Abstract
Several studies reported that natural and synthetic estrogens are the major contributors to the estrogenic activity associated with the effluents of wastewater treatment plants. The ability of the enzyme laccase to degrade these compounds in batch experiments has been demonstrated in previous studies. Nevertheless, information is scarce regarding in vitro degradation of estrogens in continuous enzymatic bioreactors. The present work constitutes an important step forward for the implementation of an enzymatic reactor for the continuous removal of estrone (E1) and estradiol (E2) by free laccase from Myceliophthora thermophila. In a first step, the effect of the main process parameters (pH, enzyme level, gas composition (air or oxygen) and estrogen feeding rate) were evaluated in fed-batch bioreactors. E1 and E2 were oxidized by 94.1 and 95.5%, respectively, under the best conditions evaluated. Thereafter, an enzymatic membrane reactor (EMR) was developed to perform the continuous degradation of the estrogens. The configuration consisted of a stirred tank reactor coupled with an ultrafiltration membrane, which allowed the recovery of enzyme while both estrogens and degradation products could pass through it. The highest removal ra...Continue Reading
References
Nov 1, 1993·Trends in Biotechnology·E Katchalski-Katzir
Feb 24, 1999·The Science of the Total Environment·T A TernesJ Mueller
Mar 10, 2001·Enzyme and Microbial Technology·A BódaloM C. Montiel
Oct 19, 2002·Journal of Biotechnology·C LópezJ M Lema
Apr 17, 2003·Water Research·Kazutaka SuzukiTomoaki Nishida
Nov 19, 2003·Biotechnology Advances·Dirk WesenbergSpiros N Agathos
Aug 27, 2005·Bioresource Technology·Young-Jin Kim, James A Nicell
Oct 20, 2005·Journal of Bioscience and Bioengineering·T TanakaM Taniguchi
Apr 6, 2006·Chemosphere·Yuuki TamagawaTomoaki Nishida
Jun 26, 2007·Water Research·Muriel AuriolCraig D Adams
Jun 26, 2007·Chemosphere·Daniele BilaMárcia Dezotti
Sep 11, 2007·Journal of Hazardous Materials·Santiago EsplugasMárcia Dezotti
Sep 28, 2007·Chemosphere·Muriel AuriolBenjamin Piña
Nov 13, 2007·Journal of Hazardous Materials·Paqui Blánquez, Benoit Guieysse
Feb 13, 2008·Chemosphere·J P GhoshN Biswas
Mar 20, 2008·Applied Biochemistry and Biotechnology·Atika OudiaRogério Simões
May 23, 2008·Bioresource Technology·D Moldes, T Vidal
Sep 3, 2008·Chemosphere·Patrick MazellierJoseph De Laat
Nov 21, 2008·Microbial Cell Factories·Adinarayana KunamneniMiguel Alcalde
Mar 31, 2009·Bioresource Technology·Hubert CabanaJ Peter Jones
Jun 23, 2009·Journal of Biotechnology·Meenu ChhabraTrichur Ramaswamy Sreekrishnan
Jun 23, 2009·Water Research·Krishna P KaturiP N Sarma
Jul 25, 2009·Environmental Pollution·Tomás CajthamlTomás Rezanka
Dec 3, 2010·Biodegradation·Carla NicolucciNadia Diano
Jan 18, 2011·Water Research·Hector A GarciaDesmond F Lawler
Mar 19, 2014·Environmental Science and Pollution Research International·Otilia Bizerea Spiridon, Laura Pitulice
Citations
Apr 3, 2013·Environmental Science & Technology·Lucia LloretJuan M Lema
Nov 2, 2014·Applied Microbiology and Biotechnology·Christoph A GasserPhilippe F-X Corvini
Oct 9, 2014·Membranes·Matthias de CazesJosé Sanchez-Marcano
Jul 16, 2014·Critical Reviews in Biotechnology·Bijender Singh
Jun 3, 2015·Biotechnology Progress·Roberto Taboada-PuigJuan M Lema
Sep 15, 2014·Journal of Hazardous Materials·Sidy BaHubert Cabana
Sep 21, 2013·Bioresource Technology·Luong N NguyenLong D Nghiem
May 5, 2016·Environmental Technology·J BlavierF Debaste
Jun 27, 2017·Journal of Environmental Management·Muhammad B AsifLong D Nghiem
Dec 29, 2017·Critical Reviews in Biotechnology·Jagdeep SinghRajeev Kumar Kapoor
Nov 9, 2018·Applied Microbiology and Biotechnology·Abha SharmaR C Kuhad
Dec 8, 2017·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Bingjie XuChangchao Zhan
Jul 26, 2015·Applied Microbiology and Biotechnology·A Arca-RamosM T Moreira
May 23, 2019·3 Biotech·Seema Dahiya, Bijender Singh
Feb 5, 2021·Journal of Hazardous Materials·Qingzhu LiuYoubin Si
Jan 11, 2018·New Biotechnology·Daniela HuberGeorg M Guebitz