Degradation of estrogens by laccase from Myceliophthora thermophila in fed-batch and enzymatic membrane reactors

Journal of Hazardous Materials
L LloretJ M Lema

Abstract

Several studies reported that natural and synthetic estrogens are the major contributors to the estrogenic activity associated with the effluents of wastewater treatment plants. The ability of the enzyme laccase to degrade these compounds in batch experiments has been demonstrated in previous studies. Nevertheless, information is scarce regarding in vitro degradation of estrogens in continuous enzymatic bioreactors. The present work constitutes an important step forward for the implementation of an enzymatic reactor for the continuous removal of estrone (E1) and estradiol (E2) by free laccase from Myceliophthora thermophila. In a first step, the effect of the main process parameters (pH, enzyme level, gas composition (air or oxygen) and estrogen feeding rate) were evaluated in fed-batch bioreactors. E1 and E2 were oxidized by 94.1 and 95.5%, respectively, under the best conditions evaluated. Thereafter, an enzymatic membrane reactor (EMR) was developed to perform the continuous degradation of the estrogens. The configuration consisted of a stirred tank reactor coupled with an ultrafiltration membrane, which allowed the recovery of enzyme while both estrogens and degradation products could pass through it. The highest removal ra...Continue Reading

References

Nov 1, 1993·Trends in Biotechnology·E Katchalski-Katzir
Nov 19, 2003·Biotechnology Advances·Dirk WesenbergSpiros N Agathos
Aug 27, 2005·Bioresource Technology·Young-Jin Kim, James A Nicell
Nov 13, 2007·Journal of Hazardous Materials·Paqui Blánquez, Benoit Guieysse
Nov 21, 2008·Microbial Cell Factories·Adinarayana KunamneniMiguel Alcalde
Jun 23, 2009·Journal of Biotechnology·Meenu ChhabraTrichur Ramaswamy Sreekrishnan
Jul 25, 2009·Environmental Pollution·Tomás CajthamlTomás Rezanka
Jan 18, 2011·Water Research·Hector A GarciaDesmond F Lawler

❮ Previous
Next ❯

Citations

Nov 2, 2014·Applied Microbiology and Biotechnology·Christoph A GasserPhilippe F-X Corvini
Oct 9, 2014·Membranes·Matthias de CazesJosé Sanchez-Marcano
Jun 3, 2015·Biotechnology Progress·Roberto Taboada-PuigJuan M Lema
Dec 29, 2017·Critical Reviews in Biotechnology·Jagdeep SinghRajeev Kumar Kapoor
Nov 9, 2018·Applied Microbiology and Biotechnology·Abha SharmaR C Kuhad
Dec 8, 2017·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Bingjie XuChangchao Zhan
Jan 11, 2018·New Biotechnology·Daniela HuberGeorg M Guebitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.