Degradation of extracellular polymeric substances (EPS) extracted from activated sludge by low-concentration ozonation

Chemosphere
Lu MengMarvin Yeung

Abstract

Reaction mechanisms between ozone and extracellular polymeric substances (EPS) can be the key of understanding the improvements in microbial aggregates properties by low-concentration ozonation. In this study, EPS are extracted from activated sludge and treated continuously by ozone gas at 270 ± 41 ppm. The reaction between ozone and EPS was investigated by observation of EPS component concentrations, functional groups and molecular weight distributions using UV-Vis spectrometry, excitation-emission matrix fluorescence spectroscopy (EEM), high performance size-exclusion chromatography (HPSEC) and gas chromatography-mass spectrometry (GC-MS). In a 12-hour-ozonation experiment, significant ozone consumption was observed in the first 4 h and protein concentration in EPS solution was reduced by 30 ± 12%. However, the polysaccharides concentration only had a slightly decrease at the end of the ozonation process. UV-Vis spectra and EEM spectra results suggest that ozone removed protein and fluorescent matters (SMP and tryptophan-like aromatic protein) rapidly by attacking specific amino acid residues on polypeptide chain. After ozonation, the molecular weight of polysaccharide and protein dropped by 4 orders of magnitude according to...Continue Reading

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Citations

May 28, 2020·Environmental Science and Pollution Research International·Lingxiao WangYanfang Liu
Nov 1, 2020·Environmental Science and Pollution Research International·Clara V FariaFabiana V Fonseca
Nov 10, 2020·Environmental Technology·Alessandro di BiaseJan A Oleszkiewicz
Mar 4, 2021·Water Environment Research : a Research Publication of the Water Environment Federation·Beiying LiBin Ma
May 8, 2021·Water Research·J Rajesh BanuGopalakrishnan Kumar

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