Degradation of hammerhead ribozymes by human ribonucleases

Molecular & General Genetics : MGG
L QiuK Drlica

Abstract

Hammerhead ribozymes were used as substrates to examine endoribonucleolytic activities in cell extracts and cultured human cells. Primer-extension analyses showed that ribozymes directed against tumor necrosis factor-alpha mRNA and human immunodeficiency virus type 1 tat mRNA were cleaved at UA and CA dinucleotides by extracts. Preferred cleavage sites were similar to those observed following digestion with RNase A, and cleavage was blocked by RNasin, an inhibitor of pyrimidine-specific ribonucleases. Removal of UA and CA dinucleotides rendered ribozymes more stable when incubated in cell extracts that were not significantly contaminated by extracellular nucleases. Placement of UA dinucleotides adjacent to a ribozyme in mRNA led to excision of the ribozyme from long transcripts during incubation in extracts. UA dinucleotides also made mRNA more labile than a control RNA when expressed from an endogenous plasmid gene in the human myeloid cell line U937. Similarly, UA and CA dinucleotides caused ribozymes to have a shorter half-life when delivered to U937 cells by lipofectin-mediated transformation. Taken together, these data indicate that one or more members of the pyrimidine-specific ribonuclease family is involved in the intra...Continue Reading

Citations

Feb 18, 2010·The Journal of Biological Chemistry·Krzysztof SobczakWlodzimierz J Krzyzosiak
Jan 5, 2006·Applied and Environmental Microbiology·Karine FiolaBenoit Cousineau
Oct 2, 2014·European Journal of Human Genetics : EJHG·Ting WangJoe Gn Garcia
Apr 28, 2004·Biochemical and Biophysical Research Communications·Yu-Hui LinKin-Fu Chak
Mar 18, 2005·Molecular Biology and Evolution·David B Carlini
Jan 28, 2006·Journal of Cellular Biochemistry·Wei-Hua Pan, Gary A Clawson
Oct 8, 2016·Preparative Biochemistry & Biotechnology·R SaravananAshok Kumar Dubey

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