PMID: 8586270Dec 15, 1995Paper

Degradation of homovanillate by a strain of Variovorax paradoxus via ring hydroxylation

FEMS Microbiology Letters
N AllisonR Wait

Abstract

A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalysed a typical monooxygenase reaction and had little activity towards 4-hydroxyphenylacetic acid GC-MS and TLC analysis suggested that homovanillate was 1-hydroxylated to yield a dihydroxymonomethoxyphenylacetic acid which served as a substrate for homogentisate 1,2-dioxygenase. Methanol, but not formaldehyde, was released either during ring-cleavage or subsequent metabolism of the ring-cleavage product.

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Citations

Nov 30, 2012·Applied Microbiology and Biotechnology·Barbara SatolaAlexander Steinbüchel
Nov 22, 2016·Environmental Microbiology·Abdul SamadAngela Sessitsch
Aug 13, 2013·Antonie van Leeuwenhoek·Katarzyna Hupert-KocurekZofia Piotrowska-Seget

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