Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance

Cardiovascular Research
D DouY Gao

Abstract

A depressed cGMP-dependent protein kinase (PKG) activity is implicated in nitrate tolerance. The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance. Nitrate tolerance in in vitro preparations was obtained by a 24 h incubation with nitroglycerin (NTG). Nitrate tolerance in in vivo preparations was obtained by subcutaneous injection of mice with NTG, and the aortas were used. Protein levels of total MYPT1, MYPT1 (LZ+), PP1Cdelta, myosin light chain (MLC), and phosphorylated MLC were determined by Western blot analysis. Isometric vessel tension was determined by an organ chamber technique. Protein levels of MYPT1 (LZ+), but not of PP1Cdelta, were significantly reduced in in vitro and in vivo nitrate-tolerant arteries. The decrease in the MYPT1 (LZ+) protein level of coronary artery was also induced by a nitric oxide donor and a cGMP analogue, which was prevented by the inhibitors of soluble guanylyl cyclase and PKG. The decrease in MYPT1 (LZ+) protein levels was not affected by the inhibitor of protein synthesis, but was prevented by the inhibitors of proteasomes. The dim...Continue Reading

References

Oct 31, 1989·Biochemical and Biophysical Research Communications·M E GoldL J Ignarro
Jan 1, 1989·Journal of Molecular and Cellular Cardiology·C Romanin, W R Kukovetz
Jan 1, 1994·Annual Review of Physiology·S H Francis, J D Corbin
Oct 19, 1993·European Journal of Pharmacology·E D PaganiP J Silver
Jun 23, 1998·Journal of Muscle Research and Cell Motility·D J HartshorneF Erdödi
Oct 28, 1998·Trends in Cell Biology·D H Lee, A L Goldberg
May 12, 2000·British Journal of Pharmacology·G Abou-MohamedR W Caldwell
Aug 4, 2001·The Journal of Biological Chemistry·J J KhatriS A Fisher
Jan 30, 2002·British Journal of Pharmacology·Ellen Q WangHo-Leung Fung
Feb 27, 2003·Clinical Pharmacokinetics·Satoru Hashimoto, Atsuko Kobayashi
Oct 8, 2003·The Journal of Biological Chemistry·Qi Quan HuangFrank V Brozovich
Oct 24, 2003·Molecular Pharmacology·Jon DiFabioBrian M Bennett
Feb 11, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Verena StanglKarl Stangl
Aug 24, 2004·Circulation Research·Syed M KarimFrank V Brozovich
Jan 1, 2005·Pharmacology & Therapeutics·Tamás Csont, Péter Ferdinandy
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhiqiang ChenJonathan S Stamler
Oct 1, 2005·Circulation Research·Thomas MünzelAlexander Mülsch
Dec 7, 2005·The Journal of Pharmacology and Experimental Therapeutics·Jeffery D MacPhersonBrian M Bennett
Dec 17, 2005·Journal of Molecular and Cellular Cardiology·Michael C PayneSteven A Fisher
Dec 24, 2005·Physiological Reviews·F HofmannJ Schlossmann
Jul 4, 2006·Journal of Molecular and Cellular Cardiology·Frank C ChenFrank V Brozovich
Oct 24, 2006·Trends in Cardiovascular Medicine·Zhiqiang Chen, Jonathan S Stamler
Feb 13, 2007·Circulation Research·Haiying Zhang, Steven A Fisher
Apr 10, 2009·PloS One·Erhan Ararat, Frank V Brozovich

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Citations

Apr 27, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Huijuan MaYuansheng Gao
Nov 23, 2012·Journal of Vascular Research·Young Soo Han, Frank V Brozovich
Nov 30, 2011·Circulation Journal : Official Journal of the Japanese Circulation Society·Dou DouYuansheng Gao
Oct 29, 2013·Journal of Molecular and Cellular Cardiology·Ewa A KonikFrank V Brozovich
Apr 14, 2012·Circulation Journal : Official Journal of the Japanese Circulation Society·Dou DouYuansheng Gao
Aug 30, 2014·Archives of Biochemistry and Biophysics·Samantha L YuenFrank V Brozovich
Oct 25, 2011·Pflügers Archiv : European journal of physiology·Lei YingYuansheng Gao
Dec 13, 2012·Journal of Cardiovascular Pharmacology·Dou DouYuansheng Gao
Mar 25, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Qiuyu YaoHongfang Jin
Jun 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Jens Schlossmann, Matthias Desch
Jun 15, 2011·Chemical Reviews·Achala de MelAlexander M Seifalian
Oct 7, 2021·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Yiqin CuiYan Yang

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