Degradation of levan by Actinomyces viscosus.

Infection and Immunity
C H Miller, P J Somers

Abstract

Actinomyces viscosus ATCC 15987 was examined for its ability to hydrolyze its own levan. Washed whole cells and an ammonium sulfate fraction from cell-free culture fluids were shown to possess levan hydrolase activity. Analyses of reaction mixtures by gel filtration and thin-layer chromatography demonstrated that the product of levan hydrolysis was free fructose. The cell-associated and extracellular enzyme preparations also hydrolyzed inulin and the levans synthesized by Aerobacter levanicum and Bacillus subtilis. Growth of A. viscosus in media supplemented with 0.1% A. viscosus levan resulted in a 33-fold increase and a 7-fold increase in the specific activities of the respective extracellular and cell-associated enzymes when compared with those from 55 mM glucose cultures. Growth in the presence of 29.2 mM sucrose resulted in a 28-fold increase and a 5-fold increase in the specific activities of the respective enzymes when compared with those from the glucose cultures. The extracellular enzyme exhibited high activity over a wide pH range, with 87 and 89% of its pH 6.0 optimum activity at pH 5.0 and 7.0, respectively. The cell-associated enzyme also exhibited optimum activity at pH 6.0, but this was decreased to 10 and 20% at...Continue Reading

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Citations

Jan 1, 1985·Microbiology and Immunology·K TakamoriN Takahashi
Jan 18, 2011·Applied and Environmental Microbiology·Ayako OgawaHidenobu Senpuku
Jun 13, 2000·Journal of Bacteriology·L J BergeronR A Burne
Jun 12, 2009·Critical Reviews in Biotechnology·Krishnan VijayaraghavanN Sravya Sowdamini
May 1, 1995·Applied and Environmental Microbiology·E WankerH Schwab
Dec 1, 1987·Infection and Immunity·T IgarashiK Takamori
Oct 1, 1983·Infection and Immunity·N TakahashiK Takamori

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