PMID: 9435074Jan 22, 1998Paper

Degradation of morpholine by an environmental Mycobacterium strain involves a cytochrome P-450

Applied and Environmental Microbiology
P PoupinA M Delort

Abstract

A Mycobacterium strain (RP1) was isolated from a contaminated activated sludge collected in a wastewater treatment unit of a chemical plant. It was capable of utilizing morpholine and other heterocyclic compounds, such as pyrrolidine and piperidine, as the sole source of carbon, nitrogen, and energy. The use of in situ 1H nuclear magnetic resonance (1H NMR) spectroscopy allowed the determination of two intermediates in the biodegradative pathway, 2-(2-aminoethoxy)acetate and glycolate. The inhibitory effects of metyrapone on the degradative abilities of strain RP1 indicated the involvement of a cytochrome P-450 in the biodegradation of morpholine. This observation was confirmed by spectrophotometric analysis and 1H NMR. Reduced cell extracts from morpholine-grown cultures, but not succinate-grown cultures, gave rise to a carbon monoxide difference spectrum with a peak near 450 nm, which indicated the presence of a soluble cytochrome P-450. 1H NMR allowed the direct analysis of the incubation medium containing metyrapone, a specific inhibitor of cytochrome P-450. The inhibition of morpholine degradation was dependent on the morpholine/metyrapone ratio. The heme-containing monooxygenase was also detected in pyrrolidine- and piper...Continue Reading

References

Sep 1, 1992·Journal of Bacteriology·J S UotilaM S Salkinoja-Salonen
Sep 1, 1991·Molecular Microbiology·D P O'Keefe, P A Harder
Jan 1, 1991·Methods in Enzymology·J A Peterson, J Y Lu
Jan 1, 1991·Advances in Applied Microbiology·F S Sariaslani
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·F P Guengerich
May 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F P Guengerich, T L MacDonald
Feb 1, 1988·Applied and Environmental Microbiology·J S CechJ Chudoba
Jan 1, 1981·Drug Metabolism Reviews·B Testa, P Jenner
Sep 1, 1994·Canadian Journal of Microbiology·N Mazure, N Truffaut
Jun 1, 1996·Molecular Microbiology·A W Munro, J G Lindsay
Mar 1, 1996·Microbiological Reviews·G F WhiteE C Tidswell
Oct 1, 1996·Applied Microbiology and Biotechnology·D Dean-Ross, C E Cerniglia
Jan 22, 1998·Applied and Environmental Microbiology·P PoupinA M Delort
Jan 1, 1990·Environmental Pollution·J S Knapp, A J Whytell

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Citations

Dec 23, 2006·Journal of Industrial Microbiology & Biotechnology·M D AdjeiJ B Sutherland
Apr 12, 2000·Trends in Biotechnology·L Brecker, D W Ribbons
Dec 5, 2000·Current Opinion in Biotechnology·H Weber, L Brecker
Jan 22, 1998·Applied and Environmental Microbiology·P PoupinA M Delort
May 3, 2013·Applied Microbiology and Biotechnology·Kathleen E DuncanKerry Sublette
Nov 27, 2002·Applied and Environmental Microbiology·Nicolas HarouneAnne-Marie Delort
Jul 4, 2003·Applied and Environmental Microbiology·Joanna D MoodyCarl E Cerniglia
Feb 8, 2006·Applied and Environmental Microbiology·Sugima RappertRudolf Müller
Nov 14, 2008·Mikrobiologiia·I T ErmakovaA A Leont'evskiĭ
Jul 22, 2017·SAR and QSAR in Environmental Research·N H DimitrovaC Hamaguchi
Jun 11, 2003·Canadian Journal of Microbiology·M TriguiD Thomas
Apr 4, 2006·Microbiological Research·Aruliah RajasekarAnnamalai Rajendran

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