Degradation of phosphate polymer polyP enhances lactic fermentation in mice

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Akihiro NakamuraKenji Miyado

Abstract

In bacteria, a polymer of inorganic phosphate (Pi) (inorganic polyphosphate; polyP) is enzymatically produced and consumed as an alternative phosphate donor for adenosine triphosphate (ATP) production to protect against nutrient starvation. In vertebrates, polyP has been dismissed as a "molecular fossil" due to the lack of any known physiological function. Here, we have explored its possible role by producing transgenic (TG) mice widely expressing Saccharomyces cerevisiae exopolyphosphatase 1 (ScPPX1), which catalyzes hydrolytic polyP degradation. TG mice were produced and displayed reduced mitochondrial respiration in muscles. In female TG mice, the blood concentration of lactic acid was enhanced, whereas ATP storage in liver and brain tissues was reduced significantly. Thus, we suggested that the elongation of polyP reduces the intracellular Pi concentration, suppresses anaerobic lactic acid production, and sustains mitochondrial respiration. Our results provide an insight into the physiological role of polyP in mammals, particularly in females.

References

Aug 1, 1980·Canadian Journal of Microbiology·R A Allan, J J Miller
Mar 17, 1995·The Journal of Biological Chemistry·K D Kumble, A Kornberg
Aug 17, 1999·Progress in Molecular and Subcellular Biology·A Kornberg
Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·A SethuramanA Kornberg
Sep 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lihong WangRichard A Roth
Feb 27, 2007·Fungal Genetics and Biology : FG & B·Thomas P WernerFlorian M Freimoser
Sep 7, 2007·Molecular Biology of the Cell·Hans Caspar HürlimannFlorian M Freimoser
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrey Y AbramovEvgeny Pavlov
Aug 15, 2008·Biochemistry·Marko TammenkoskiReijo Lahti
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kenji MiyadoAkihiro Umezawa
Apr 7, 2009·Annual Review of Biochemistry·Narayana N RaoArthur Kornberg
Feb 4, 2010·The Journal of Biological Chemistry·Evgeny PavlovAndrey Y Abramov
Mar 19, 2011·BMC Research Notes·Shun-Ichiro OguraTohru Tanaka
Aug 6, 2011·Microbial Cell Factories·Lucia Achbergerová, Jozef Nahálka
Feb 11, 2016·Biochemical Society Transactions·Plamena R AngelovaAndrey Y Abramov
Feb 11, 2016·Biochemical Society Transactions·Sidney OmelonWouter Habraken
Aug 16, 2017·Journal of Basic Microbiology·Ludmila TrilisenkoTatiana Kulakovskaya
Nov 2, 2017·Biochemistry and Biophysics Reports·Antasar AliShamus Burns

❮ Previous
Next ❯

Citations

Apr 10, 2020·The Biochemical Journal·Artyom Y BaevAndrey Y Abramov
Oct 29, 2020·Cell Reports·Emma Bondy-ChorneyMichael Downey
Jun 21, 2021·The Journal of Biological Chemistry·Naoya SawadaKojiro Takeda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.