PMID: 2499360Jun 27, 1989Paper

Degradation of plasma bilirubin by a bilirubin oxidase derivative which has a relatively long half-life in the circulation

Biochimica Et Biophysica Acta
K SugiY Morino

Abstract

To enhance degradation of unconjugated bilirubin in hyperbilirubinemic subjects, we synthesized a bilirubin oxidase (EC 1.3.3.5) (BO) derivative (PEGBO) by covalently linking (2,4-bis[O-methoxy(polyethyleneglycol)]-6-chloro-s-triazine) (PEG) to the enzyme. Intravenously injected BO in rats disappeared from the circulation with a half-life of 2.5 min; the half-life of PEGBO was 190 min. Intravenously injected BO minimally and transiently decreased plasma bilirubin levels in jaundiced Gunn rats and in bile-duct-ligated jaundiced rats. In contrast, PEGBO rapidly and substantially decreased plasma bilirubin levels and the effect persisted for longer than 3 h. Renal dysfunction often occurs in patients with liver diseases. To study the role of bilirubin toxicity for the kidney, functions of transtubular transport for organic anions was measured in bile-duct-ligated jaundiced animals before and after treatment with PEGBO. Bile duct ligation decreased urinary excretion of phenolsulfophthalein (PSP), an organic anion used for renal function test. Treatment of the jaundiced animals with PEGBO increased the rate of PSP disappearance from the circulation and normalized its urinary excretion. Thus, PEGBO might be useful for the study of bi...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T R SkopekW G Thilly
May 1, 1987·Journal of General Microbiology·D S Plaha, H J Rogers
Sep 1, 1987·British Journal of Industrial Medicine·S ShimaY Yamamoto
Jan 1, 1970·Advances in Clinical Chemistry·T Peters
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A G BraunB B Nichinson

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Citations

Apr 1, 1994·Journal of Protein Chemistry·T SamejimaJ T Yang
Oct 1, 1994·Journal of Gastroenterology·M HirotaM Inoue
Apr 10, 2008·Journal of Clinical Biochemistry and Nutrition·Ji-Young HongMasayasu Inoue
Sep 26, 2000·Molecular Genetics and Metabolism·T W Hansen
Dec 28, 1989·Biochimica Et Biophysica Acta·K SugiT Sato

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