Degradation of polyubiquitinated cyclin B is blocked by the MAPK pathway at the metaphase I arrest in starfish oocytes.

The Journal of Biological Chemistry
Eiko OitaKazuyoshi Chiba

Abstract

In the starfish ovary, maturing oocytes stimulated by 1-methyladenine undergo synchronous germinal vesicle breakdown and then arrest in metaphase of the first meiotic division (metaphase I). Immediately after spawning, an increase of intracellular pH (pH(i)) from approximately 7.0 to approximately 7.3 is induced by Na(+)/H(+) antiporter in oocytes, and meiosis reinitiation occurs. Here we show that an endogenous substrate of the proteasome, polyubiquitinated cyclin B, was stable at pH 7.0, whereas it was degraded at pH 7.3. When the MAPK pathway was blocked by MEK inhibitor U0126, degradation of polyubiquitinated cyclin B occurred even at pH 7.0 without an increase of the peptidase activity of the proteasome. These results indicate that the proteasome activity at pH 7.0 is sufficient for degradation of polyubiquitinated cyclin B and that the MAPK pathway blocks the degradation of polyubiquitinated cyclin B in the maturing oocytes in the ovary. Immediately after spawning, the increase in pH(i) mediated by Na(+)/H(+) antiporter cancels the inhibitory effects of the MAPK pathway, resulting in the degradation of polyubiquitinated cyclin B and the release of the arrest. Thus, the key step of metaphase I arrest in starfish oocytes oc...Continue Reading

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Citations

May 29, 2010·The Journal of Biological Chemistry·Kaori HaradaKazuyoshi Chiba
Apr 12, 2014·Biochemical and Biophysical Research Communications·Vlad CostacheRémi Dumollard
Aug 11, 2004·Developmental Biology·Ri-ko Matsuura, Kazuyoshi Chiba
Sep 21, 2019·The Journal of Cell Biology·Enako HosodaKazuyoshi Chiba
Nov 4, 2006·The Journal of Biological Chemistry·Qiang ZhaoZihe Rao
Feb 26, 2020·Cells·Kazuyoshi Chiba

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