PMID: 6111031Sep 1, 1980Paper

Degradation of prolylleucylglycinamide (MIF) by mouse brain

Neurochemical Research
A NeidleA Lajtha

Abstract

Prolylleucylglycinamide (MIF) at 1.0 mM concentration and pH 7.0 was hydrolyzed by mouse brain homogenate at a rate of 140 nmol/mg protein/hr. Nearly all of this activity can be accounted for by the action of two enzymes, both of which cleave Pro and Leu sequentially from the N-terminus of MIF. At pH 7.0 the predominant enzyme is arylamidase, inhibited by puromycin (1 mM) and Mn2+ (2.5 mM). At pH 8.5, in the presence of Mn2+, a second enzyme with a higher potential activity (570 nmol/mg protein/hr) was observed. While the arylamidase is primarily localized in the cytosol, the Mn2+-stimulated enzyme is equally divided between soluble and particulate fractions. Because of its ability to cleave leucinamide, its high pH optimum, and its Mn2+ dependence, it can be classified as a leucine aminopeptidase (LAP). In its substrate specifically and its preference for Mn2+ over Mg2+ it resembles the LAP from connective tissue more than that from other sources.

References

Jul 11, 1979·Biochimica Et Biophysica Acta·H P SchnebliR K Barclay
Jul 30, 1979·Life Sciences·A J KastinD H Coy
Jan 1, 1971·Experientia·L AbrashN Marks
Jun 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·N Marks, R Walter
Jun 18, 1971·Biochemical and Biophysical Research Communications·R M NairA V Schally
Feb 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·N MarksR Walter
Aug 29, 1973·Journal of Chromatography·M Roth, A Hampaï
Apr 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A Nordwig, H Mayer
Jun 1, 1971·Canadian Journal of Biochemistry·R E Sobel, A S Brecher
Apr 1, 1969·The Biochemical Journal·A S Brecher, J B Suszkiw
Jul 1, 1963·Biochemistry·M L KORNGUTHH WAELSCH

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Citations

Jan 1, 1984·Neurochemistry International·N MarksD H Coy
Aug 1, 1985·Neurochemical Research·K S HuiA Lajtha
May 1, 1982·The Journal of Pharmacy and Pharmacology·S L Dickinson, P Slater
Jan 1, 1984·Journal of Neuroscience Research·M J Berg, N Marks

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