Degradation of raw feather by a novel high molecular weight extracellular protease from newly isolated Bacillus cereus DCUW.

Journal of Industrial Microbiology & Biotechnology
Abhrajyoti GhoshDhrubajyoti Chattopadhyay

Abstract

Biotreatment of feather wastes and utilization of the degraded products in feed and foodstuffs has been a challenge. In the present study, we have demonstrated the degradation of feather waste by Bacillus cereus DCUW strain isolated during a functional screening based microbial diversity study on East Calcutta Wetland Area. A high molecular weight keratinolytic protease from feather degrading DCUW strain was purified and characterized. Moreover, utilization of degraded products during feather hydrolysis was developed and demonstrated. The purified keratinolytic protease was found to show pH and temperature optima of 8.5 and 50 degrees C, respectively. PMSF was found to inhibit the enzyme completely. The purified enzyme showed molecular weight of 80 kDa (from SDS-PAGE). The protease was found to have broad range substrate specificities that include keratin, casein, collagen, fibrin, BAPNA and gelatin. The protease was identified as minor extracellular protease (Vpr) by RT-PCR and northern blotting techniques. This is the first report describing the characterization of minor extracellular protease (Vpr) and its involvement in feather degradation in B. cereus group of organisms.

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Citations

Dec 30, 2009·Applied Microbiology and Biotechnology·Adriano BrandelliAlessandro Riffel
Feb 21, 2009·Journal of Industrial Microbiology & Biotechnology·Nereida Mello Rosa da GioppoRosane M Peralta
Jun 1, 2011·World Journal of Microbiology & Biotechnology·Ana Maria MazottoAlane B Vermelho
Nov 27, 2015·Systematic and Applied Microbiology·Bastian HerzogRussell G Kerr
Jun 14, 2016·Critical Reviews in Biotechnology·Amit VermaGurpreet Singh Dhilon
Jan 14, 2010·Prikladnaia biokhimiia i mikrobiologiia·N L EremeevO V Koroleva
Mar 21, 2020·International Journal of Biological Macromolecules·Mohamed A HassanEman Abbas
Apr 28, 2021·Current Microbiology·Ranjeeta BhariRam Sarup Singh

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