Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13: identification of a new biglycan cleavage site

Arthritis Research & Therapy
Jordi MonfortJohanne Martel-Pelletier

Abstract

A major and early feature of cartilage degeneration is proteoglycan breakdown. Matrix metalloprotease (MMP)-13 plays an important role in cartilage degradation in osteoarthritis (OA). This MMP, in addition to initiating collagen fibre cleavage, acts on several proteoglycans. One of the proteoglycan families, termed small leucine-rich proteoglycans (SLRPs), was found to be involved in collagen fibril formation/interaction, with some members playing a role in the OA process. We investigated the ability of MMP-13 to cleave members of two classes of SLRPs: biglycan and decorin; and fibromodulin and lumican. SLRPs were isolated from human normal and OA cartilage using guanidinium chloride (4 mol/l) extraction. Digestion products were examined using Western blotting. The identities of the MMP-13 degradation products of biglycan and decorin (using specific substrates) were determined following electrophoresis and microsequencing. We found that the SLRPs studied were cleaved to differing extents by human MMP-13. Although only minimal cleavage of decorin and lumican was observed, cleavage of fibromodulin and biglycan was extensive, suggesting that both molecules are preferential substrates. In contrast to biglycan, decorin and lumican, ...Continue Reading

References

Dec 1, 1992·Matrix : Collagen and Related Research·D H VyniosC P Tsiganos
Mar 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P Witsch-PrehmH Kresse
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, U Lindahl
Jul 15, 1989·The Biochemical Journal·L I Melching, P J Roughley
Jan 1, 1982·Connective Tissue Research·R W FarndaleA J Barrett
Jan 1, 1983·Arthritis and Rheumatism·J P PelletierJ F Woessner
Sep 1, 1995·The Journal of Biological Chemistry·L SvenssonA Oldberg
Sep 15, 1995·The Journal of Biological Chemistry·J GroverP J Roughley
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·H HedlundO Svensson
Jan 19, 1996·The Journal of Biological Chemistry·V KnäuperG Murphy
Feb 12, 1996·FEBS Letters·A J FosangP J Neame
Sep 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A R PooleP J Roughley
Apr 1, 1997·The Journal of Clinical Investigation·R C BillinghurstA R Poole
Nov 14, 1997·Neuroscience Research·R Vigot, C Batini
Jun 17, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·A M HockingD J McQuillan
Aug 29, 1998·The Journal of Biological Chemistry·P LorenzoD Heinegård
Oct 6, 1998·Annual Review of Biochemistry·R V Iozzo
Apr 9, 2001·Seminars in Cell & Developmental Biology·C B Knudson, W Knudson
Jul 11, 2001·The Journal of Biological Chemistry·B ManssonD Heinegård
Feb 16, 2002·The Journal of Biological Chemistry·Eva BengtssonAnders Aspberg
Apr 5, 2002·Journal of Inorganic Biochemistry·Kanehisa KojohTakayoshi Wakagi
Dec 10, 2003·The Journal of Biological Chemistry·Masahide KashiwagiHideaki Nagase
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paul G ScottJordi Bella
Aug 5, 2005·Plastic and Reconstructive Surgery·Sarper Yílmaz

❮ Previous
Next ❯

Citations

Sep 28, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·James MelroseChristopher B Little
Nov 19, 2010·The Journal of Clinical Investigation·Kristin MorethLiliana Schaefer
Feb 8, 2011·Reproductive Biology and Endocrinology : RB&E·Renato M SalgadoTelma M T Zorn
Jul 24, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Madalina V NastaseLiliana Schaefer
Oct 18, 2013·International Journal of Molecular Sciences·Mandy J PeffersPeter D Clegg
Jan 16, 2010·Connective Tissue Research·Liang ZhangGary Gibson
May 15, 2012·Cell Cycle·Kristin MorethLiliana Schaefer
Mar 19, 2015·Advances in Wound Care·Margaret Mary Smith, James Melrose
Sep 26, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Anuradha KrishnanMichael Charlton
Apr 26, 2014·Journal of the American Society of Nephrology : JASN·Hans-Joachim Anders, Liliana Schaefer
Jul 1, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mandy Jayne PeffersPeter David Clegg
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Renáta GáspárAnikó Görbe
Nov 15, 2008·The Journal of Biological Chemistry·Sebastian Kalamajski, Åke Oldberg
Dec 18, 2009·Journal of Cellular and Molecular Medicine·Andrea MarzollJens W Fischer
Dec 26, 2015·Arthritis Research & Therapy·Goncalo BarretoKari K Eklund
Jan 11, 2020·Frontiers in Pharmacology·Chenshuang LiMin Zou
Jan 13, 2018·Scientific Reports·Aina FarránJohanne Martel-Pelletier
Oct 22, 2020·Clinical Science·Kaj E C BloklandJanette K Burgess
Oct 10, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tai-Wei ZhangLi-Bo Jiang
Nov 2, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Michele ScuruchiGiuseppe Mandraffino
May 6, 2008·Best Practice & Research. Clinical Rheumatology·Johanne Martel-PelletierPeter J Roughley
Sep 15, 2011·Biochimica Et Biophysica Acta·Heather StantonAmanda J Fosang
Aug 13, 2021·The Indian Journal of Medical Research·Kenan Ozler

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Methods Mentioned

BETA
electrophoresis
amino acid sequencing

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