Degradation of the benzoxazolinone class of phytoalexins is important for virulence of Fusarium pseudograminearum towards wheat

Molecular Plant Pathology
Andrew J KettleDonald M Gardiner

Abstract

Wheat, maize, rye and certain other agriculturally important species in the Poaceae family produce the benzoxazolinone class of phytoalexins on pest and pathogen attack. Benzoxazolinones can inhibit the growth of pathogens. However, certain fungi can actively detoxify these compounds. Despite this, a clear link between the ability to detoxify benzoxazolinones and pathogen virulence has not been shown. Here, through comparative genome analysis of several Fusarium species, we have identified a conserved genomic region around the FDB2 gene encoding an N-malonyltransferase enzyme known to be involved in benzoxazolinone degradation in the maize pathogen Fusarium verticillioides. Expression analyses demonstrated that a cluster of nine genes was responsive to exogenous benzoxazolinone in the important wheat pathogen Fusarium pseudograminearum. The analysis of independent F. pseudograminearum FDB2 knockouts and complementation of the knockout with FDB2 homologues from F. graminearum and F. verticillioides confirmed that the N-malonyltransferase enzyme encoded by this gene is central to the detoxification of benzoxazolinones, and that Fdb2 contributes quantitatively to virulence towards wheat in head blight inoculation assays. This cont...Continue Reading

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Citations

Dec 19, 2015·The New Phytologist·Jana SperschneiderJennifer M Taylor
Feb 22, 2018·The ISME Journal·Emile Gluck-Thaler, Jason C Slot
Nov 15, 2018·Molecular Ecology·Emile Gluck-ThalerJason C Slot
May 28, 2019·The New Phytologist·Lamprinos FrantzeskakisRalph Panstruga
Mar 28, 2018·American Journal of Psychoanalysis·Merle Molofsky
Oct 5, 2017·Frontiers in Microbiology·Minglu GaoScott E Gold
May 31, 2019·Journal of Experimental Botany·Geoffrey WagnerAntoine Gravot
Nov 2, 2019·World Journal of Microbiology & Biotechnology·Vasiliki GarefalakiSotiria Boukouvala
May 18, 2021·Frontiers in Plant Science·Nathaniel M WestrickMehdi Kabbage

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