Degradation of the cartilage collagen matrix associated with changes in chondrocytes in osteoarthrosis. Assessment by loss of background fluorescence and immunodetection of matrix components

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
G J GibsonD L Lin

Abstract

Articular cartilage damage and eventual loss is the primary pathological change seen in osteoarthrosis (OA). In this study we have investigated the link between turnover of the collagen matrix and changes in chondrocytes. The background fluorescence of articular cartilage, as indicated by its emission spectrum and resistance to extraction was generated by the slow non-enzymic modification of the collagen matrix by advanced glycation end products (AGEs). Assessment of changes in background fluorescence in sections of articular cartilage provided a narrative of collagen degradation. Patients without OA pathology typically had a uniform strong background fluorescence throughout the depth of the cartilage. Cartilage from OA patients showed a range of changes in background fluorescence dependent on depth from the articular surface and proximity to overt lesions. Loss of background fluorescence was centered on chondrocytes, more extensive near the surface and associated with detection of the proteoglycan epitope 7D4. Expression of type X collagen was seen in articular cartilage in the region of the interface of with subchondral bone in most OA patients but was not associated with prominent, pericellular, loss of background fluorescen...Continue Reading

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Citations

Oct 14, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S Y TangD Vashishth
Feb 20, 2003·Current Rheumatology Reports·Deborah M Saudek, Jonathan Kay
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Dec 14, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K SubburajS Majumdar
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Aug 24, 2018·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Yoshihiko SatakeShinro Takai
Nov 27, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Allen H LinJeffrey A Weiss
Sep 10, 2003·Current Opinion in Rheumatology·Nicole VerzijlJeroen DeGroot

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