Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events.

The Journal of Biological Chemistry
Guo-Chiuan HungHui-Ling Chiang

Abstract

The key gluconeogenic enzyme fructose-1,6-bisphosphatase (FBPase) is subjected to catabolite inactivation and degradation when glucose-starved cells are replenished with fresh glucose. In various studies, the proteasome and the vacuole have each been reported to be the major site of FBPase degradation. Because different growth conditions were used in these studies, we examined whether variations in growth conditions could alter the site of FBPase degradation. Here, we demonstrated that FBPase was degraded outside the vacuole (most likely in the proteasome), when glucose was added to cells that were grown in low glucose media for a short period of time. By contrast, cells that were grown in the same low glucose media for longer periods of time degraded FBPase in the vacuole in response to glucose. Another gluconeogenic enzyme malate dehydrogenase (MDH2) showed the same degradation characteristics as FBPase in that the short term starvation of cells led to a non-vacuolar degradation, whereas long term starvation resulted in the vacuolar degradation of this protein. The N-terminal proline is required for the degradation of FBPase and MDH2 for both the vacuolar and non-vacuolar proteolytic pathways. The cAMP signaling pathway and t...Continue Reading

References

Sep 1, 1990·Microbiological Reviews·D J KlionskyS D Emr
Aug 1, 1971·Journal of Bacteriology·C Gancedo
Dec 1, 1994·Archives of Biochemistry and Biophysics·K I Minard, L McAlister-Henn
Jun 1, 1993·Journal of Bacteriology·D CliftonD G Fraenkel
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Feb 24, 1997·The Journal of Cell Biology·P H Huang, H L Chiang
May 8, 1997·Nature·B J NicholsA Haas
Aug 1, 1997·Molecular Biology of the Cell·M J De VitM Johnston
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·K LudinM Carlson
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·J M Gancedo
Oct 17, 1998·Molecular and Cellular Biology·M A TreitelM Carlson
Mar 24, 1999·Trends in Genetics : TIG·M Johnston
May 14, 1999·Current Opinion in Microbiology·M Carlson
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M BochtlerR Huber
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C UngermannW Wickner
Aug 31, 2000·Annual Review of Biochemistry·J Kim, D J Klionsky
Dec 16, 2000·The EMBO Journal·G EitzenW Wickner
May 10, 2001·Trends in Biochemical Sciences·F RollandJ M Thevelein
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·H Abeliovich, D J Klionsky
Oct 20, 2001·The Journal of Biological Chemistry·C R BrownH L Chiang
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·James A FlemingRonald K Blackman
Mar 13, 2002·The EMBO Journal·William Wickner

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Citations

Mar 5, 2013·Current Genetics·J Horák
May 1, 2012·Molecular & Cellular Proteomics : MCP·Niclas OlssonChrister Wingren
Sep 19, 2007·Molecular and Cellular Biology·Nitnipa SoontorngunBernard Turcotte
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·George M Santangelo
Jul 10, 2010·International Journal of Biological Sciences·Yan Yan, Bin Kang
Feb 14, 2014·Proteome Science·Bennett J GiardinaHui-Ling Chiang
Mar 31, 2009·International Journal of Molecular Sciences·Yumiko IwahashiHitoshi Iwahashi
Sep 13, 2015·Critical Reviews in Biochemistry and Molecular Biology·Kunio NakatsukasaTakumi Kamura
Jun 2, 2010·Biochemical and Biophysical Research Communications·Jeannette JuretschkeDieter H Wolf
Mar 9, 2010·Biochemical and Biophysical Research Communications·Lise BarbinDieter H Wolf
May 20, 2015·Molecular Cell·Kunio NakatsukasaTakumi Kamura
Jun 19, 2008·FEMS Microbiology Reviews·Juana M Gancedo
Sep 12, 2012·Autophagy·Daniel J KlionskyBrian Zuckerbraun
Aug 19, 2009·FEMS Yeast Research·Bernard TurcotteNitnipa Soontorngun
May 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jang-Hyun OhAlexander Varshavsky
Oct 19, 2017·DNA and Cell Biology·Chelsea M WintersHui-Ling Chiang
Nov 30, 2011·World Journal of Biological Chemistry·Abbas A Alibhoy, Hui-Ling Chiang
Nov 11, 2017·The Journal of Biological Chemistry·Jang-Hyun OhAlexander Varshavsky
Jun 11, 2009·Communicative & Integrative Biology·C Randell Brown, Hui-Ling Chiang
Mar 21, 2019·Biological Chemistry·Huaize Liu, Thorsten Pfirrmann

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