Degradation of transgenic Cry1Ab DNA and protein in Bt-176 maize during the ensiling process

Journal of Animal Physiology and Animal Nutrition
Bodo LutzChristiane Albrecht

Abstract

Maize silage is commonly used as feed for farm animals. The aim of this study was to monitor the time-dependent degradation of non-recombinant chloroplast DNA (exemplified by the rubisco gene) in comparison with the recombinant cry1Ab gene in the course of the ensiling process. In parallel, the Cry1Ab protein content and fragment sizes were determined. Fragments of the rubisco (173, 896, 1197, 1753 and 2521 bp) and of the cry1Ab gene (211, 420, 727 and 1,423 bp) were selected to investigate the DNA degradation process. The detection of the Cry1Ab protein was performed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Rubisco gene fragments of 173 bp were still detectable after 61 days, while fragments of 1,197 and 2,521 bp were detectable up to 30 days and on the first day only respectively. Polymerase chain reaction (PCR) analyses revealed that fragments of the cry1Ab gene with sizes of 211 and 420 bp were detectable up to 61 days, fragments with sizes of 727 and 1,423 bp, 30 and 6 days respectively. The ELISA showed a decrease of the Cry1Ab protein in maize silage during the ensiling process. No marked degradation was observed during the first 43 h. Thereafter, a sharp decrease was measured. After 61 days...Continue Reading

References

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Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Johann de Vries, Wilfried Wackernagel
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Oct 9, 2003·Applied and Environmental Microbiology·Mitra KharazmiChristian Hertel
Feb 13, 2004·The Lancet Infectious Diseases·Claudia Orellana
Mar 3, 2005·Journal of Agricultural and Food Chemistry·Bodo LutzChristiane Albrecht

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Citations

Dec 17, 2009·Analytical and Bioanalytical Chemistry·Nicolas Gryson
Oct 16, 2007·Applied and Environmental Microbiology·S WiedemannC Albrecht
Jun 29, 2010·Journal of Animal Physiology and Animal Nutrition·K SteinkeF J Schwarz
Jul 21, 2017·Journal of Animal Science·A L Van Eenennaam, A E Young
Oct 21, 2009·Journal of Dairy Science·E PonzoniD Breviario

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