Degradation of Trichloroethylene by Methanol-Grown Cultures of Methylosinus trichosporium OB3b PP358.

Applied and Environmental Microbiology
M W FitchG Georgiou

Abstract

A soluble methane monooxygenase-constitutive mutant strain of Methylosinus trichosporium OB3b, strain PP358, was grown with methanol as the carbon source, and the kinetics of trichloroethylene (TCE) degradation were determined. PP358 exhibited high TCE degradation rates under both oxygen- and carbon-limiting conditions. The optimal pseudo first-order rate constant for TCE was comparable to the values measured for cells grown with methane. We found that growth under oxygen-limiting conditions results in increased accumulation of polyhydroxybutyrate, which in turn correlates with higher transformation capacities for TCE. It was also shown that methanol inhibits TCE degradation only at high concentrations. Thus, methanol-grown cultures of PP358 represent an efficient system for the biodegradation of chlorinated hydrocarbons.

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Citations

Jan 15, 1999·Critical Reviews in Microbiology·J P SullivanH A Chase
Oct 13, 2012·Critical Reviews in Biotechnology·Awadhesh Kumar ShuklaSuresh Kumar Dubey
Nov 9, 2005·Water Environment Research : a Research Publication of the Water Environment Federation·Jonathan G PressmanGerald E Speitel
Jul 5, 2011·Applied and Environmental Microbiology·Allison J PiejaCraig S Criddle
Jan 27, 2021·Environmental Science and Pollution Research International·Jofre HerreroJosé M Carmona
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Feb 13, 2001·Journal of Biotechnology·J E van Hylckama Vlieg, D B Janssen
Mar 15, 2006·Applied and Environmental Microbiology·V J Van HylckamaD B Janssen
Nov 10, 2021·The Science of the Total Environment·Diana PuigserverJosé M Carmona

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