DegS is necessary for virulence and is among extraintestinal Escherichia coli genes induced in murine peritonitis.

Infection and Immunity
P RedfordR A Welch

Abstract

Extraintestinal Escherichia coli strains cause meningitis, sepsis, urinary tract infection, and other infections outside the bowel. We examined here extraintestinal E. coli strain CFT073 by differential fluorescence induction. Pools of CFT073 clones carrying a CFT073 genomic fragment library in a promoterless gfp vector were inoculated intraperitoneally into mice; bacteria were recovered by lavage 6 h later and then subjected to fluorescence-activated cell sorting. Eleven promoters were found to be active in the mouse but not in Luria-Bertani (LB) broth culture. Three are linked to genes for enterobactin, aerobactin, and yersiniabactin. Three others are linked to the metabolic genes metA, gltB, and sucA, and another was linked to iha, a possible adhesin. Three lie before open reading frames of unknown function. One promoter is associated with degS, an inner membrane protease. Mutants of the in vivo-induced loci were tested in competition with the wild type in mouse peritonitis. Of the mutants tested, only CFT073 degS was found to be attenuated in peritoneal and in urinary tract infection, with virulence restored by complementation. CFT073 degS shows growth similar to that of the wild type at 37 degrees C but is impaired at 43 d...Continue Reading

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Citations

May 23, 2006·Nature Reviews. Microbiology·Gary RowleyMark Roberts
May 13, 2009·The Journal of Infectious Diseases·Eric L BucklesMichael S Donnenberg
Jun 30, 2007·Foodborne Pathogens and Disease·James L SmithNereus W Gunther
May 7, 2008·Infection and Immunity·Richard R KulesusMatthew A Mulvey
Jun 23, 2006·Infection and Immunity·Peter Redford, Rodney A Welch
Oct 27, 2004·Infection and Immunity·Jennifer A SnyderHarry L T Mobley
Oct 22, 2005·Infection and Immunity·Jennifer A SnyderHarry L T Mobley
Oct 22, 2005·Infection and Immunity·M Chelsea LaneHarry L T Mobley
Mar 14, 2007·Journal of Bacteriology·Amanda L LloydHarry L T Mobley
Apr 6, 2006·Journal of Bacteriology·Balakrishnan VenkateshShinji Tsuyumu
Sep 6, 2003·American Journal of Respiratory and Critical Care Medicine·Jin-Hwa LeeHaibo Zhang
Jun 8, 2010·BMC Microbiology·Carla Th P HopmanYvonne Pannekoek
Dec 13, 2005·PLoS Biology·Virgil A RhodiusCarol A Gross
Dec 18, 2013·PLoS Pathogens·Sargurunathan SubashchandraboseHarry L T Mobley
Aug 22, 2009·Research in Microbiology·Michael MeltzerMichael Ehrmann
Apr 23, 2004·Molecular Microbiology·Benjamin M Alba, Carol A Gross
Jan 5, 2020·Journal of Biomedical Science·Wen-Chun HuangChing-Hao Teng
Sep 26, 2019·Frontiers in Cellular and Infection Microbiology·Claire L HewsTracy L Raivio
Dec 24, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Takehiro KadoShunji Ueno
Aug 1, 2009·EcoSal Plus·Dawn M Macritchie, Tracy L Raivio
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Sep 10, 2015·Microbiology Spectrum·Sargurunathan Subashchandrabose, Harry L T Mobley
Jan 1, 2006·EcoSal Plus·Jay L Mellies, Alex M S Barron

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