Deguelin inhibits epithelial-to-mesenchymal transition and metastasis of human non-small cell lung cancer cells by regulating NIMA-related kinase 2

Thoracic Cancer
Dejian ZhaoYu Chen

Abstract

Non-small cell lung cancer is a lethal malignancy with a high mortality rate. Deguelin displays an anti-tumor effect and inhibits metastasis in various cancers. The aberrant expression of NIMA-related kinase 2 (NEK2) indicates poor prognosis and induces epithelial-to-mesenchymal transition (EMT) and metastasis processes. However, the underlying mechanism between deguelin and NEK2 has remained elusive. NSCLC cell lines were treated with deguelin. Wound-healing and invasion assays were applied to study the inhibitory effect of deguelin on NSCLC cells. EMT markers, E-cadherin and Vimentin, were also detected by Western blot. NEK2 protein and messenger RNA expression levels were evaluated when NSCLC cells were treated with different concentrations of deguelin. The effect of NEK2 on NSCLC cell metastasis was evaluated through NEK2 knockdown. To investigate whether deguelin induced EMT by regulating NEK2, we overexpressed NEK2 in both NCI-H520 and SK-MES-1 cell lines, and then used real time-PCR to study the E-cadherin and Vimentin messenger RNA expression in both NSCLC cells. Deguelin inhibited migration and invasion processes in NSCLC cell lines and decreased NEK2 expression in a concentration-dependent manner. Furthermore, NEK2 kn...Continue Reading

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Citations

Dec 1, 2017·International Journal of Molecular Sciences·Marilina García-Aranda, Maximino Redondo
Oct 3, 2019·Experimental Biology and Medicine·Shirley Jusino, Harold I Saavedra
Oct 5, 2019·Anti-cancer Agents in Medicinal Chemistry·Mehmet A KocdorHilal Kocdor
Feb 14, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hardeep Singh TuliAnupam Bishayee

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Methods Mentioned

BETA
electrophoresis
transfection
PCR

Software Mentioned

SPSS

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