Dehydration of biological membranes by cooling: an investigation on the purple membrane

Journal of Molecular Biology
Ruep E LechnerThomas Hauss

Abstract

The lamellar spacing dl of purple membrane (PM) multilayer systems was investigated with neutron diffraction as a function of temperature and of the level of hydration. The observed large T-dependent variations of dl indicate that PM is partially dehydrated when cooled below a "hydration water freezing point". This phenomenon is reversible, but a hysteresis is observed when PM is rehydrated upon reheating. The hydration water remaining bound to the membrane below about 240 K is non-freezing. Its amount was found to be hnf=0.24(+/-0.02) g 2H2O/g BR for all samples equilibrated at room temperature in the presence of 2H2O vapour at >/=84% r.h. It is evident, that the dehydration/rehydration behaviour of PM is strongly correlated with the temperature-dependent behaviour of the dynamical structure factor. Above the well-known "dynamical transition" announcing the onset of localized diffusive molecular motions between 190 K and 230 K, a second dynamical transition is caused by the temperature-induced rehydration of the PM starting near 255 K. This is also correlated with the deviation from a pure Arrhenius law of the rate-limiting process in the photocycle, known to occur upon cooling beyond the ice point into the same temperature re...Continue Reading

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Citations

Feb 21, 2008·European Biophysics Journal : EBJ·K WoodM Weik
Sep 30, 2008·Biochemistry·Andrei K Dioumaev, Janos K Lanyi
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·K WoodM Weik
Jan 13, 2017·The Journal of Physical Chemistry. B·V N Syryamina, S A Dzuba
Jun 21, 2008·Journal of Molecular Biology·K WoodG Zaccai
Aug 2, 2005·Biophysical Journal·Peter BerntsenJan Swenson
Aug 11, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Maikel C RheinstädterTim Salditt
Feb 21, 2009·Faraday Discussions·Douglas J TobiasMounir Tarek
Jul 7, 2020·Frontiers in Chemistry·Jakob MeinekeGiovanna Fragneto
Mar 15, 2008·Biophysical Journal·Kathleen WoodDieter Oesterhelt

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