Dehydration-responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation-mediated regulation of stress response

Plant, Cell & Environment
Pragya BaruaNiranjan Chakraborty

Abstract

Nonavailability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NPs) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration, and NPs were extracted from unstressed control and from 72- and 144-hr stressed tissues. We identified 4,832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases, and transcription factors, besides 660 uncharacterized proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho-motif analysis revealed stress-induced enrichment of proline-directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated protein...Continue Reading

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Citations

Dec 24, 2018·Plant, Cell & Environment·Christine H FoyerHon-Ming Lam
Jul 31, 2020·BMC Genomics·Saeedreza VessalKadambot H M Siddique
Dec 8, 2020·The Plant Journal : for Cell and Molecular Biology·Divya MishraNiranjan Chakraborty
Oct 19, 2019·Plant Science : an International Journal of Experimental Plant Biology·Akanksha PareekNiranjan Chakraborty
May 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Pallavi SinhaRajeev K Varshney
May 29, 2021·Biological Reviews of the Cambridge Philosophical Society·Mohammad ArefianT S Keshava Prasad

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