Dehydration-stress-regulated transgene expression in stably transformed rice plants

Plant Physiology
J SuR Wu

Abstract

To confer abscisic acid (ABA) and/or stress-inducible gene expression, an ABA-response complex (ABRC1) from the barley (Hordeum vulgare L.) HVA22 gene was fused to four different lengths of the 5' region from the rice (Oryza sativa L.) Act1 gene. Transient assay of beta-glucuronidase (GUS) activity in barley aleurone cells shows that, coupled with ABRC1, the shortest minimal promoter (Act1-100P) gives both the greatest induction and the highest level of absolute activity following ABA treatment. Two plasmids with one or four copies of ABRC1 combined with the same Act1-100P and HVA22(I) of barley HVA22 were constructed and used for stable expression of uidA in transgenic rice plants. Three Southern blot-positive lines with the correct hybridization pattern for each construct were obtained. Northern analysis indicated that uidA expression is induced by ABA, water-deficit, and NaCl treatments. GUS activity assays in the transgenic plants confirmed that the induction of GUS activity varies from 3- to 8-fold with different treatments or in different rice tissues, and that transgenic rice plants harboring four copies of ABRC1 show 50% to 200% higher absolute GUS activity both before and after treatments than those with one copy of AB...Continue Reading

References

Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G GiulianoA R Cashmore
Jan 1, 1995·Plant Physiology·EAH. Pilon-SmitsSCM. Smeekens
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·J. Ingram, D. Bartels
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N K SinghR A Bressan
Oct 29, 1982·Science·J S Boyer
Aug 1, 1989·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·X ZhaoR Wu
Dec 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W Zhang, R Wu

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Citations

Apr 12, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Benze XiaoLizhong Xiong
Apr 12, 2001·Plant Science : an International Journal of Experimental Plant Biology·M Roy, R Wu
Sep 26, 2013·Planta·Kazuo NakashimaKazuko Yamaguchi-Shinozaki
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ajay K GargRay J Wu
Nov 15, 2011·Plant Physiology and Biochemistry : PPB·Meng CuiRay Wu
Jul 3, 2007·Journal of Plant Physiology·Eliane Cristina Gruszka VendruscoloLuiz Gonzaga Esteves Vieira
Jan 27, 2011·Plant Biotechnology Journal·Jacqueline FreemanHuw D Jones
Sep 7, 2014·Molecular Biology Reports·Satish K GuttikondaHenry T Nguyen

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