Dehydrin MtCAS31 promotes autophagic degradation under drought stress.

Autophagy
Xin LiTao Wang

Abstract

Drought stress seriously affects crop yield, and the mechanism underlying plant resistance to drought stress via macroautophagy/autophagy is not clear. Here, we show that a dehydrin, Medicago truncatula MtCAS31 (cold acclimation-specific 31), a positive regulator of drought response, plays a key role in autophagic degradation. A GFP cleavage assay and treatment with an autophagy-specific inhibitor indicated that MtCAS31 participates in the autophagic degradation pathway and that overexpressing MtCAS31 promotes autophagy under drought stress. Furthermore, we discovered that MtCAS31 interacts with the autophagy-related protein ATG8a in the AIM-like motif YXXXI, supporting its function in autophagic degradation. In addition, we identified a cargo protein of MtCAS31, the aquaporin MtPIP2;7, by screening an M. truncatula cDNA library. We found that MtPIP2;7 functions as a negative regulator of drought response. Under drought stress, MtCAS31 facilitated the autophagic degradation of MtPIP2;7 and reduced root hydraulic conductivity, thus reducing water loss and improving drought tolerance. Taken together, our results reveal a novel function of dehydrins in promoting the autophagic degradation of proteins, which extends our knowledge o...Continue Reading

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Citations

Mar 19, 2020·Frontiers in Plant Science·Tong SuPingping Wang
Sep 4, 2020·International Journal of Molecular Sciences·Jie RanJian-Zhong Liu
Jul 3, 2020·Plants·Lynnette M A DirkAllan Bruce Downie
Dec 2, 2020·International Journal of Molecular Sciences·Wanlong SuWeilun Yin
Jan 28, 2021·International Journal of Molecular Sciences·Shuwei LuoZhixiang Chen
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Liuqing HuoFengwang Ma
May 1, 2021·International Journal of Molecular Sciences·Hong ChenTao Wang
Sep 2, 2021·The Plant Journal : for Cell and Molecular Biology·Jie Tang, Diane C Bassham
Sep 17, 2021·Frontiers in Plant Science·Xiuling ChenGuangkun Yin

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Methods Mentioned

BETA
protein folding
transgenic
confocal microscopy
cleavage assay
two-hybrid
coimmunoprecipitation
Bimolecular
fluorescence complementation assay
Co-IP
environmental stress

Software Mentioned

SPSS
ImageJ

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