Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants

Frontiers in Plant Science
Tanmoy HalderSudipta Ray

Abstract

Environmental stresses generate reactive oxygen species (ROS) which might be detrimental to the plants when produced in an uncontrolled way. However, the plants ameliorate such stresses by synthesizing antioxidants and enzymes responsible for the dismutation of ROS. Additionally, the dehydrins were also able to protect the inactivation of the enzyme lactate dehydrogenase against hydroxyl radicals (OH⋅) generated during Fenton's reaction.SbDhn1andSbDhn2overexpressing transgenic tobacco plants were able to protect against oxidative damage. Transgenic tobacco lines showed better photosynthetic efficiency along with high chlorophyll content, soluble sugar and proline. However, the malonyl dialdehyde (MDA) content was significantly lower in transgenic lines. Experimental evidence demonstrates the protective effect of dehydrins on electron transport chain in isolated chloroplast upon methyl viologen (MV) treatment. The transgenic tobacco plants showed significantly lower superoxide radical generation () upon MV treatment. The accumulation of the H2O2was also lower in the transgenic plants. Furthermore, in the transgenic plants the expression of ROS scavenging enzymes was higher compared to non-transformed (NT) or vector transformed (...Continue Reading

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Citations

May 7, 2019·Frontiers in Plant Science·Ana Paula Zotta MotaAna Cristina Miranda Brasileiro
Dec 15, 2020·Plant Physiology and Biochemistry : PPB·María Vazquez-HernandezMaría Isabel Escribano

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Methods Mentioned

BETA
environmental stress
transgenic
column chromatography
PCR
MDA
electron transport
Fluorescence
Assay

Software Mentioned

Biolyzer
Primer3

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