Dehydroalanine crosslinks in human lens

Experimental Eye Research
M LinetskyF Hu

Abstract

This study was conducted to develop a methodology for the purification and detection of histidinoalanine, lanthionine and lysinoalanine in the lens tissue. Cataractous and aged human lens proteins were hydrolysed and fractionated by using anion-exchange chromatography. The fraction containing the bulk of dehydroalanine crosslinks was derivatized with dansyl chloride and then separated and quantified by means of RP-HPLC. The spectral and chromatographic properties of all three substances purified and quantified in this study were identical to those of their synthesized counterparts. Histidinoalanine and lanthionine were the most abundant dehydroalanine crosslinks in both water-soluble and water-insoluble lens proteins. Histidinoalanine levels in water-soluble proteins from the cataractous lenses of Indian origin were 6.2-fold higher than those in water-soluble proteins from normal lenses (1.68+/-0.75 vs 0.26+/-0.06 nmol/mg protein; p<0.001). In water-insoluble proteins, they were 2.2-fold higher in cataractous lenses compared with normal lenses (1.59+/-0.76 vs 0.73+/-0.17 nmol/mg protein; p<0.01). Lanthionine levels were significantly higher in water-insoluble proteins of cataractous lenses when compared to non-cataractous lense...Continue Reading

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Citations

Aug 9, 2011·Amino Acids·Ine RomboutsJan A Delcour
May 3, 2005·Experimental Eye Research·Roger J W Truscott
Jul 27, 2014·Genome Biology and Evolution·Shradha Khater, Debasisa Mohanty
Apr 30, 2013·The Journal of Biological Chemistry·Qingchun ZhangGregory C Flynn
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Oct 22, 2013·The Journal of Biological Chemistry·Qingchun Zhang, Gregory C Flynn
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Nov 11, 2019·Progress in Retinal and Eye Research·Kevin L ScheyRoger J W Truscott
May 16, 2007·Journal of the American Chemical Society·Steven L CohenJosef Vlasak
Apr 15, 2021·Expert Review of Proteomics·Lee S Cantrell, Kevin L Schey
Jun 21, 2021·Experimental Eye Research·Kevin L ScheyRoger J W Truscott
Mar 28, 2019·The Journal of Physical Chemistry. B·Tomoki NakayoshiAkifumi Oda
Jul 15, 2005·Analytical Chemistry·Jonathan A Karty, James P Reilly
Oct 23, 2021·ACS Chemical Biology·Michael G FriedrichRoger J W Truscott

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