Dehydroascorbate: a possible surveillance molecule of oxidative stress and programmed cell death in the green alga Chlamydomonas reinhardtii.

The New Phytologist
Omer MurikAaron Kaplan

Abstract

Chlamydomonas reinhardtii tolerates relatively high H2 O2 levels that induce an array of antioxidant activities. However, rather than rendering the cells more resistant to oxidative stress, the cells become far more sensitive to an additional H2 O2 dose. If H2 O2 is provided 1.5-9 h after an initial dose, it induces programmed cell death (PCD) in the wild-type, but not in the dum1 mutant impaired in the mitochondrial respiratory complex III. This mutant does not exhibit a secondary oxidative burst 4-5 h after the inducing H2 O2 , nor does it activate metacaspase-1 after the second H2 O2 treatment. The intracellular dehydroascorbate level, a product of ascorbate peroxidase, increases under conditions leading to PCD. The addition of dehydroascorbate induces PCD in the wild-type and dum1 cultures, but higher levels are required in dum1 cells, where it is metabolized faster. The application of dehydroascorbate induces the expression of metacaspase-2, which is much stronger than the expression of metacaspase-1. The presence or absence of oxidative stress, in addition to the rise in internal dehydroascorbate, may determine which metacaspase is activated during Chlamydomonas PCD. Cell death is strongly affected by the timing of H2 O2 ...Continue Reading

References

Feb 1, 1992·Journal of General Microbiology·L P Collinson, I W Dawes
Jan 1, 1984·Methods in Enzymology·H Aebi
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·K Lewis
Jan 23, 2002·Cell Death and Differentiation·N LeeH L Nakhasi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 20, 2002·Cell Death and Differentiation·E V Koonin, L Aravind
Aug 13, 2002·Current Genetics·Frank MadeoKai-Uwe Fröhlich
Mar 14, 2003·Analytical Biochemistry·Toyoki AmanoYuzo Shioi
Dec 11, 2003·The Journal of Biological Chemistry·Irene BaroliKrishna K Niyogi
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Krishna K. Niyogi
Sep 24, 2004·The Journal of Cell Biology·Silke WissingFrank Madeo
Oct 12, 2004·Proceedings. Biological Sciences·Daniel J Franklin, John A Berges
Nov 5, 2004·Developmental Cell·J Marie Hardwick, Wen-Chih Cheng
Mar 6, 2007·Molecular and Biochemical Parasitology·Ludovic Le ChatJohannes T Dessens
Mar 16, 2007·Environmental Microbiology·Daniella SchatzAaron Kaplan
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Tobias EisenbergFrank Madeo
Sep 22, 2007·Chembiochem : a European Journal of Chemical Biology·Angelo FontanaAdrianna Ianora
Oct 13, 2007·Science·Sabeeha S MerchantArthur R Grossman
Oct 31, 2007·The Journal of Cell Biology·Dominique VercammenFrank Van Breusegem
Feb 23, 2008·Biochimica Et Biophysica Acta·Marcel Deponte
Feb 26, 2009·Antioxidants & Redox Signaling·Christine H Foyer, Graham Noctor
Jul 7, 2009·Annual Review of Plant Biology·Christine H FoyerGraham Noctor
Feb 2, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Irina V Shemarova
Feb 16, 2010·Cell Death and Differentiation·D Carmona-GutierrezF Madeo
Aug 21, 2010·Evolution; International Journal of Organic Evolution·Aurora M NedelcuArmin Rashidi
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sandy VanderauweraRon Mittler

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Citations

Oct 8, 2015·Trends in Plant Science·Olivier Van Aken, Frank Van Breusegem
Aug 28, 2014·Journal of Biotechnology·Lutz Wobbe, Claire Remacle
Jul 13, 2016·Current Biology : CB·Kay D Bidle
Sep 12, 2018·Bioscience Reports·Véronique Larosa, Claire Remacle
Aug 8, 2019·Environmental Microbiology Reports·Einat DanielAaron Kaplan
Aug 7, 2021·Toxins·Leyberth José Fernández-HerreraMauricio Muñoz-Ochoa
Jul 11, 2021·The Plant Journal : for Cell and Molecular Biology·Tsubasa SodeyamaTakahiro Ishikawa
Jul 29, 2021·FEBS Letters·Katarina Petra van MiddenMarina Klemenčič

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